Rapid recruitment of late endosomes and lysosomes in mouse macrophages ingesting Candida albicans

Rita Káposzta, L. Máródi, Michael Hollinshead, Siamon Gordon, Rosângela P. Da Silva

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Candida albicans is an important opportunistic pathogen, whose interaction with cells of the immune system, in particular macrophages (M∅), is poorly understood. In order to learn more about the nature of tile infectious mechanism, internalisation of Candida albicans was studied in mouse M∅ by confocal immunofluorescence and electron microscopy in comparison with latex beads of similar size, which were coated with mannosyl-lipoarabinomannan (ManLAM) to target the M∅ mannose receptor (MR). Uptake of Candida yeasts characteristics of phagocytosis, required intact filaments, and depended on the activity of protein kinase C (PKC). Candida phagosomes rapidly attracted lysosome-associated membrane protein (Lamp)-rich vacuoles, indicative of fusion with late endosomes and lysosomes. Rapid recruitment of late endosomes and lysosomes could be observed regardless of heat-inactivation or serum-opsonisation of Candida, but did not follow binding of the mannosylated-beads to M∅, which suggest that this phenotype is not MR-specific. The yeasts developed germ tubes within phagolysosomes, distended their membranes and escaped, destroying the non-activated M∅. The filamentous form of Candida could penetrate intact M∅ even when phagocytosis was blocked, and also attracted Lamp-rich organelles. Inhibition of lysosomal acidification and associated lysosomal fusion reduced germ tube formation of Candida within the phagolysosomes. These data suggest that rapid recruitment of late endocytic/lysosomal compartments by internalising C. albicans favours survival and virulence of this pathogen.

Original languageEnglish
Pages (from-to)3237-3248
Number of pages12
JournalJournal of Cell Science
Volume112
Issue number19
Publication statusPublished - 1999

Fingerprint

Endosomes
Lysosomes
Candida albicans
Candida
Phagosomes
Macrophages
Lysosome-Associated Membrane Glycoproteins
Phagocytosis
Yeasts
Vacuoles
Microspheres
Fluorescence Microscopy
Confocal Microscopy
Cell Communication
Organelles
Protein Kinase C
Virulence
Immune System
Electron Microscopy
Hot Temperature

Keywords

  • Candida
  • Endosome
  • Macrophage
  • Phagocytosis
  • Phagolysosome

ASJC Scopus subject areas

  • Cell Biology

Cite this

Káposzta, R., Máródi, L., Hollinshead, M., Gordon, S., & Da Silva, R. P. (1999). Rapid recruitment of late endosomes and lysosomes in mouse macrophages ingesting Candida albicans. Journal of Cell Science, 112(19), 3237-3248.

Rapid recruitment of late endosomes and lysosomes in mouse macrophages ingesting Candida albicans. / Káposzta, Rita; Máródi, L.; Hollinshead, Michael; Gordon, Siamon; Da Silva, Rosângela P.

In: Journal of Cell Science, Vol. 112, No. 19, 1999, p. 3237-3248.

Research output: Contribution to journalArticle

Káposzta, R, Máródi, L, Hollinshead, M, Gordon, S & Da Silva, RP 1999, 'Rapid recruitment of late endosomes and lysosomes in mouse macrophages ingesting Candida albicans', Journal of Cell Science, vol. 112, no. 19, pp. 3237-3248.
Káposzta, Rita ; Máródi, L. ; Hollinshead, Michael ; Gordon, Siamon ; Da Silva, Rosângela P. / Rapid recruitment of late endosomes and lysosomes in mouse macrophages ingesting Candida albicans. In: Journal of Cell Science. 1999 ; Vol. 112, No. 19. pp. 3237-3248.
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