Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout

Bo Tian, Jing Ma, Teresa Zardán Gómez De La Torre, A. Bálint, Marco Donolato, Mikkel Fougt Hansen, Peter Svedlindh, Mattias Strömberg

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Rapid and sensitive diagnostic methods based on isothermal amplification are ideal substitutes for PCR in out-of-lab settings. However, there are bottlenecks in terms of establishing low-cost and user-friendly readout methods for isothermal amplification schemes. Combining the high amplification efficiency of loop-mediated isothermal amplification (LAMP) with an optomagnetic nanoparticle-based readout system, we demonstrate ultrasensitive and rapid detection of Newcastle disease virus RNA. Biotinylated amplicons of LAMP and reverse transcription LAMP (RT-LAMP) bind to streptavidin-coated magnetic nanoparticles (MNPs) resulting in a dramatical increase in the hydrodynamic size of the MNPs. This increase was measured by an optomagnetic readout system and provided quantitative information on the amount of LAMP target sequence. Our assay resulted in a limit of detection of 10 aM of target sequence with a total assay time of 30 min. The assay has also been tested on clinical samples (vaccine and tissue specimens) with a performance comparable to real-time RT-PCR. By changing the LAMP primers, this strategy can serve as a general method for the detection of other DNA/RNA targets with high specificity and sensitivity.

Original languageEnglish
Pages (from-to)1228-1234
Number of pages7
JournalACS Sensors
Volume1
Issue number10
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

viruses
Viruses
Amplification
readout
Readout systems
Assays
Nanoparticles
RNA
nanoparticles
vaccines
primers
Vaccines
Streptavidin
Transcription
DNA
Hydrodynamics
deoxyribonucleic acid
hydrodynamics
substitutes
Tissue

Keywords

  • loop-mediated isothermal amplification
  • magnetic nanoparticles
  • Newcastle disease virus
  • optomagnetic bioassay
  • out-of-lab diagnostics

ASJC Scopus subject areas

  • Bioengineering
  • Fluid Flow and Transfer Processes
  • Process Chemistry and Technology
  • Instrumentation

Cite this

Tian, B., Ma, J., Zardán Gómez De La Torre, T., Bálint, A., Donolato, M., Hansen, M. F., ... Strömberg, M. (2016). Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout. ACS Sensors, 1(10), 1228-1234. https://doi.org/10.1021/acssensors.6b00379

Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout. / Tian, Bo; Ma, Jing; Zardán Gómez De La Torre, Teresa; Bálint, A.; Donolato, Marco; Hansen, Mikkel Fougt; Svedlindh, Peter; Strömberg, Mattias.

In: ACS Sensors, Vol. 1, No. 10, 01.01.2016, p. 1228-1234.

Research output: Contribution to journalArticle

Tian, B, Ma, J, Zardán Gómez De La Torre, T, Bálint, A, Donolato, M, Hansen, MF, Svedlindh, P & Strömberg, M 2016, 'Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout', ACS Sensors, vol. 1, no. 10, pp. 1228-1234. https://doi.org/10.1021/acssensors.6b00379
Tian, Bo ; Ma, Jing ; Zardán Gómez De La Torre, Teresa ; Bálint, A. ; Donolato, Marco ; Hansen, Mikkel Fougt ; Svedlindh, Peter ; Strömberg, Mattias. / Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout. In: ACS Sensors. 2016 ; Vol. 1, No. 10. pp. 1228-1234.
@article{ec575a2979284dba864b2cc32f1d0cde,
title = "Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout",
abstract = "Rapid and sensitive diagnostic methods based on isothermal amplification are ideal substitutes for PCR in out-of-lab settings. However, there are bottlenecks in terms of establishing low-cost and user-friendly readout methods for isothermal amplification schemes. Combining the high amplification efficiency of loop-mediated isothermal amplification (LAMP) with an optomagnetic nanoparticle-based readout system, we demonstrate ultrasensitive and rapid detection of Newcastle disease virus RNA. Biotinylated amplicons of LAMP and reverse transcription LAMP (RT-LAMP) bind to streptavidin-coated magnetic nanoparticles (MNPs) resulting in a dramatical increase in the hydrodynamic size of the MNPs. This increase was measured by an optomagnetic readout system and provided quantitative information on the amount of LAMP target sequence. Our assay resulted in a limit of detection of 10 aM of target sequence with a total assay time of 30 min. The assay has also been tested on clinical samples (vaccine and tissue specimens) with a performance comparable to real-time RT-PCR. By changing the LAMP primers, this strategy can serve as a general method for the detection of other DNA/RNA targets with high specificity and sensitivity.",
keywords = "loop-mediated isothermal amplification, magnetic nanoparticles, Newcastle disease virus, optomagnetic bioassay, out-of-lab diagnostics",
author = "Bo Tian and Jing Ma and {Zard{\'a}n G{\'o}mez De La Torre}, Teresa and A. B{\'a}lint and Marco Donolato and Hansen, {Mikkel Fougt} and Peter Svedlindh and Mattias Str{\"o}mberg",
year = "2016",
month = "1",
day = "1",
doi = "10.1021/acssensors.6b00379",
language = "English",
volume = "1",
pages = "1228--1234",
journal = "ACS Sensors",
issn = "2379-3694",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout

AU - Tian, Bo

AU - Ma, Jing

AU - Zardán Gómez De La Torre, Teresa

AU - Bálint, A.

AU - Donolato, Marco

AU - Hansen, Mikkel Fougt

AU - Svedlindh, Peter

AU - Strömberg, Mattias

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Rapid and sensitive diagnostic methods based on isothermal amplification are ideal substitutes for PCR in out-of-lab settings. However, there are bottlenecks in terms of establishing low-cost and user-friendly readout methods for isothermal amplification schemes. Combining the high amplification efficiency of loop-mediated isothermal amplification (LAMP) with an optomagnetic nanoparticle-based readout system, we demonstrate ultrasensitive and rapid detection of Newcastle disease virus RNA. Biotinylated amplicons of LAMP and reverse transcription LAMP (RT-LAMP) bind to streptavidin-coated magnetic nanoparticles (MNPs) resulting in a dramatical increase in the hydrodynamic size of the MNPs. This increase was measured by an optomagnetic readout system and provided quantitative information on the amount of LAMP target sequence. Our assay resulted in a limit of detection of 10 aM of target sequence with a total assay time of 30 min. The assay has also been tested on clinical samples (vaccine and tissue specimens) with a performance comparable to real-time RT-PCR. By changing the LAMP primers, this strategy can serve as a general method for the detection of other DNA/RNA targets with high specificity and sensitivity.

AB - Rapid and sensitive diagnostic methods based on isothermal amplification are ideal substitutes for PCR in out-of-lab settings. However, there are bottlenecks in terms of establishing low-cost and user-friendly readout methods for isothermal amplification schemes. Combining the high amplification efficiency of loop-mediated isothermal amplification (LAMP) with an optomagnetic nanoparticle-based readout system, we demonstrate ultrasensitive and rapid detection of Newcastle disease virus RNA. Biotinylated amplicons of LAMP and reverse transcription LAMP (RT-LAMP) bind to streptavidin-coated magnetic nanoparticles (MNPs) resulting in a dramatical increase in the hydrodynamic size of the MNPs. This increase was measured by an optomagnetic readout system and provided quantitative information on the amount of LAMP target sequence. Our assay resulted in a limit of detection of 10 aM of target sequence with a total assay time of 30 min. The assay has also been tested on clinical samples (vaccine and tissue specimens) with a performance comparable to real-time RT-PCR. By changing the LAMP primers, this strategy can serve as a general method for the detection of other DNA/RNA targets with high specificity and sensitivity.

KW - loop-mediated isothermal amplification

KW - magnetic nanoparticles

KW - Newcastle disease virus

KW - optomagnetic bioassay

KW - out-of-lab diagnostics

UR - http://www.scopus.com/inward/record.url?scp=85014191168&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014191168&partnerID=8YFLogxK

U2 - 10.1021/acssensors.6b00379

DO - 10.1021/acssensors.6b00379

M3 - Article

AN - SCOPUS:85014191168

VL - 1

SP - 1228

EP - 1234

JO - ACS Sensors

JF - ACS Sensors

SN - 2379-3694

IS - 10

ER -