Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices

Violeta Ivanova, Ágnes Dörnyei, Marina Stefova, Trajće Stafilov, Borimir Vojnoski, Ferenc Kilár, L. Márk

Research output: Contribution to journalArticle

14 Citations (Scopus)


Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) as a new valuable technique has been used for qualitative screening of anthocyanins in wine and grape samples from Vranec and Merlot variety. For that purpose, different MALDI matrices have been tested, such as α-cyano-4-hydroxycinnamic acid, sinapic acid, 2,5-dihydroxybenzoic acid (2,5-DHB), and C 70 fullerene. It was observed that 2,5-DHB was superior with respect to all the matrices used in the study. Furthermore, fullerene was, for the first time, tested and used as a possible matrix for MALDI-TOF-MS identification of anthocyanins in wine and grape samples. The results obtained showed identification of aglycone forms of anthocyanins only, since the higher laser energy applied for ionization of the fullerene molecules led to great deal of fragmentation of the sugar moiety from glucosides, acetylglucoside, and p-coumaroylglucoside groups from the corresponding anthocyanin molecules. Additionally, liquid chromatography/electrospray ionization mass spectrometry has also been applied for anthocyanin characterization.

Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalFood Analytical Methods
Issue number1
Publication statusPublished - Jan 1 2011


  • 2,5-DHB
  • Anthocyanins
  • CHCA
  • Fullerene
  • Grape
  • LC-MS
  • SA
  • Wine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Applied Microbiology and Biotechnology
  • Safety, Risk, Reliability and Quality
  • Safety Research

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