Rapid high-resolution characterization of functionally important monoclonal antibody N -glycans by capillary electrophoresis

Zoltan Szabo, A. Guttman, Jonathan Bones, Barry L. Karger

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Characterization of the N-glycosylation present in the Fc region of therapeutic monoclonal antibodies requires rapid, high-resolution separation methods to guarantee product safety and efficacy during all stages of process development. Determination of fucosylated oligosaccharides is particularly important during clone selection, product characterization, and lot release as fucose has been shown to adversely affect the ability of mAbs to induce antibody dependent cellular cytotoxicity (ADCC). Here, we apply a general capillary electrophoresis optimization strategy to separate functionally relevant fucosylated and afucosylated glycans on mononclonal antibody products in the presence of several high mannose oligosaccharides. The N-glycans chosen represent those most commonly reported on CHO cell derived therapeutic antibodies. A rapid (

Original languageEnglish
Pages (from-to)5329-5336
Number of pages8
JournalAnalytical Chemistry
Volume83
Issue number13
DOIs
Publication statusPublished - Jul 1 2011

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Capillary electrophoresis
Polysaccharides
Monoclonal Antibodies
Oligosaccharides
Antibodies
Glycosylation
Fucose
Cytotoxicity
Mannose

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Rapid high-resolution characterization of functionally important monoclonal antibody N -glycans by capillary electrophoresis. / Szabo, Zoltan; Guttman, A.; Bones, Jonathan; Karger, Barry L.

In: Analytical Chemistry, Vol. 83, No. 13, 01.07.2011, p. 5329-5336.

Research output: Contribution to journalArticle

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