Rapid genotyping of genetically modified laboratory animals from whole blood samples without DNA preparation

Á Horváth, P. Sántha, V. Horváth, Nóra Török, I. Nagy, G. Jancsó, Cs Vágvölgyi, F. Somogyvári

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1 Citation (Scopus)


A new, rapid method is described which permits the genotyping of genetically modified animals from a microlitre volume of whole blood samples via one step polymerase chain reaction amplification. The major advantage of the presented method is the exclusion of a DNA preparation step, which significantly reduces the time expenditure and work load of the genetic testing. Pilot studies indicate, that this method is efficient and applicable also on tissue biopsies and larger amount of blood providing a rapid and reliable new technique over conventional genotyping approaches.

Original languageEnglish
Pages (from-to)262-265
Number of pages4
JournalActa biologica Hungarica
Issue number2
Publication statusPublished - Jun 1 2013



  • PCR
  • blood
  • direct
  • genotyping
  • knock-out animals
  • mice

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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