Rapid genotyping of genetically modified laboratory animals from whole blood samples without DNA preparation

Á Horváth, P. Sántha, V. Horváth, Nóra Török, I. Nagy, G. Jancsó, C. Vágvölgyi, F. Somogyvári

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new, rapid method is described which permits the genotyping of genetically modified animals from a microlitre volume of whole blood samples via one step polymerase chain reaction amplification. The major advantage of the presented method is the exclusion of a DNA preparation step, which significantly reduces the time expenditure and work load of the genetic testing. Pilot studies indicate, that this method is efficient and applicable also on tissue biopsies and larger amount of blood providing a rapid and reliable new technique over conventional genotyping approaches.

Original languageEnglish
Pages (from-to)262-265
Number of pages4
JournalActa Biologica Hungarica
Volume64
Issue number2
DOIs
Publication statusPublished - Jun 1 2013

Fingerprint

Genetically Modified Animals
Laboratory Animals
Animals
Blood
blood
DNA
Biopsy
animal
Polymerase chain reaction
Amplification
Genetic Testing
Tissue
Health Expenditures
Blood Volume
Workload
polymerase chain reaction
expenditure
amplification
Testing
Polymerase Chain Reaction

Keywords

  • blood
  • direct
  • genotyping
  • knock-out animals
  • mice
  • PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Rapid genotyping of genetically modified laboratory animals from whole blood samples without DNA preparation. / Horváth, Á; Sántha, P.; Horváth, V.; Török, Nóra; Nagy, I.; Jancsó, G.; Vágvölgyi, C.; Somogyvári, F.

In: Acta Biologica Hungarica, Vol. 64, No. 2, 01.06.2013, p. 262-265.

Research output: Contribution to journalArticle

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AU - Vágvölgyi, C.

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