Rapid and efficient transformation of diploid Medicago truncatula and Medicago sativa ssp. falcata lines improved in somatic embryogenesis

T. H. Trinh, P. Ratet, E. Kondorosi, P. Durand, K. Kamaté, P. Bauer, A. Kondorosi

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R 108-1 to complete plants was further improved by three successive in vitro regeneration cycles resulting in the line R 108- 1(c3). Agrobacterium tumefaciens-mediated transformation of leaf explants was carried out with promoter-gus constructs of two early nodulins (MsEnod12A and MsEnod12B) and one late nodulin (Srglb3). The transgenic plants thus produced on all explants within 3-4 months remained diploid and were fertile. This protocol appears to be the most efficient and fastest reported so far for leguminous plants.

Original languageEnglish
Pages (from-to)345-355
Number of pages11
JournalPlant Cell Reports
Volume17
Issue number5
DOIs
Publication statusPublished - Jan 1 1998

Keywords

  • M. falcata
  • M. truncatula
  • MsEnod12A
  • MsEnod12B
  • Srglb3

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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