Random access three-dimensional two-photon microscopy

Balázs Rózsa, Gergely Katona, E. Sylvester Vizi, Zoltán Várallyay, Attila Sághy, Lásló Valenta, Pál Maák, Júlia Fekete, Ákos Bányász, Robert Szipöcs

Research output: Contribution to journalArticle

27 Citations (Scopus)


We propose a two-photon microscope scheme capable of real-time, three-dimensional investigation of the electric activity pattern of neural networks or signal summation rules of individual neurons in a 0.6 mm × 0.6 mm × 0.2 mm volume of the sample. The points of measurement are chosen according to a conventional scanning two-photon image, and they are addressed by separately adjustable optical fibers. This allows scanning at kilohertz repetition rates of as many as 100 data points. Submicrometer spatial resolution is maintained during the measurement similarly to conventional two-photon microscopy.

Original languageEnglish
Pages (from-to)1860-1865
Number of pages6
JournalApplied Optics
Issue number10
Publication statusPublished - Apr 1 2007


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

Cite this

Rózsa, B., Katona, G., Vizi, E. S., Várallyay, Z., Sághy, A., Valenta, L., Maák, P., Fekete, J., Bányász, Á., & Szipöcs, R. (2007). Random access three-dimensional two-photon microscopy. Applied Optics, 46(10), 1860-1865. https://doi.org/10.1364/AO.46.001860