Raised levels of exhaled carbon monoxide are associated with an increased expression of heme oxygenase-1 in airway macrophages in asthma: A new marker of oxidative stress

I. Horváth, Louise E. Donnelly, András Kiss, Paolo Paredi, Sergei A. Kharitonov, Peter J. Barnes

Research output: Contribution to journalArticle

260 Citations (Scopus)

Abstract

Background - Chronic inflammatory diseases are associated with an increased production of oxidants. Induction of a stress protein, heme oxygenase (HO) HO-1, is a cytoprotective mechanism against oxidative cellular injury. HO-1 catabolises heme to bilirubin, free iron, and carbon monoxide (GO). Methods - Exhaled GO and sputum bilirubin levels were measured and HO- 1 protein expression in airway macrophages was determined by Western blotting in asthmatic patients as levels of oxidants are raised in asthma and may induce HO-1. Results - Exhaled GO was significantly increased in 37 non- steroid treated asthmatic patients compared with 37 healthy subjects (5.8 (95% CI 5.20 to 6.39) ppm vs 2.9 (2.51 to 3.28) ppm; p0.05). In non-treated asthmatic patients more HO-1 protein was expressed in airway macrophages than in normal subjects. Bilirubin levels in induced sputum were also higher than in normal subjects. Inhalation of heroin, a substrate for HO, significantly increased exhaled CO from 3.8 (95% CI 2.80 to 4.87) ppm to 6.7 (95% CI 4.95 to 8.38 CI) ppm (p

Original languageEnglish
Pages (from-to)668-672
Number of pages5
JournalThorax
Volume53
Issue number8
Publication statusPublished - 1998

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Heme Oxygenase-1
Carbon Monoxide
Oxidative Stress
Asthma
Macrophages
Bilirubin
Heme Oxygenase (Decyclizing)
Sputum
Oxidants
Heroin
Heat-Shock Proteins
Heme
Inhalation
Healthy Volunteers
Proteins
Chronic Disease
Iron
Western Blotting
Steroids
Wounds and Injuries

Keywords

  • Asthma
  • Exhaled carbon monoxide
  • Heme oxygenase-1
  • Inflammation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Raised levels of exhaled carbon monoxide are associated with an increased expression of heme oxygenase-1 in airway macrophages in asthma : A new marker of oxidative stress. / Horváth, I.; Donnelly, Louise E.; Kiss, András; Paredi, Paolo; Kharitonov, Sergei A.; Barnes, Peter J.

In: Thorax, Vol. 53, No. 8, 1998, p. 668-672.

Research output: Contribution to journalArticle

Horváth, I. ; Donnelly, Louise E. ; Kiss, András ; Paredi, Paolo ; Kharitonov, Sergei A. ; Barnes, Peter J. / Raised levels of exhaled carbon monoxide are associated with an increased expression of heme oxygenase-1 in airway macrophages in asthma : A new marker of oxidative stress. In: Thorax. 1998 ; Vol. 53, No. 8. pp. 668-672.
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