Ragweed pollen extract intensifies lipopolysaccharide-induced priming of NLRP3 inflammasome in human macrophages

Aliz Varga, Marietta M. Budai, Sándor Milesz, Attila Bácsi, József To double acute zsér, Sz. Benkő

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Summary: Ragweed pollen extract (RWE) possesses intrinsic NADPH oxidase activity that induces oxidative stress by initiating the production of intracellular reactive oxygen species (ROS). The ROS are important contributors to the manifestation of allergic inflammation; furthermore, concomitant exposure to an allergen and an endotoxin trigger a stronger inflammatory response. One of the main pro-inflammatory cytokines produced in inflammatory responses is interleukin-1β (IL-1β), and its production is associated with caspase-1-containing inflammasome complexes. Intracellular ROS have been implicated in NLRP3 inflammasome-mediated IL-1β production, therefore, we aimed to study whether RWE influences the function of NLRP3 inflammasome. Here we describe that, in the presence of NADPH, RWE significantly elevates lipopolysaccharide-induced IL-1β production of THP-1 cells as well as human primary macrophages and dendritic cells. We also demonstrate that increased IL-1β production is mediated through NLRP3 inflammasome in THP-1 macrophages. We provide evidence that RWE elevates cytosolic ROS level in these cells, and ROS inhibitors abolish IL-1β production. Furthermore, we show that RWE enhances lipopolysaccharide-induced gene transcription/expression of pro-IL-1β and key components of the inflammasome via a ROS-dependent mechanism.

Original languageEnglish
Pages (from-to)392-401
Number of pages10
JournalImmunology
Volume138
Issue number4
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Inflammasomes
Interleukin-1
Lipopolysaccharides
Reactive Oxygen Species
Macrophages
Caspase 1
NADPH Oxidase
NADP
Endotoxins
Allergens
Dendritic Cells
ragweed pollen
Oxidative Stress
Cytokines
Inflammation
Gene Expression

Keywords

  • Interleukin-1β
  • NADPH oxidases
  • NLRP3 inflammasome
  • Ragweed pollen extract
  • Reactive oxygen species

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Ragweed pollen extract intensifies lipopolysaccharide-induced priming of NLRP3 inflammasome in human macrophages. / Varga, Aliz; Budai, Marietta M.; Milesz, Sándor; Bácsi, Attila; To double acute zsér, József; Benkő, Sz.

In: Immunology, Vol. 138, No. 4, 04.2013, p. 392-401.

Research output: Contribution to journalArticle

Varga, Aliz ; Budai, Marietta M. ; Milesz, Sándor ; Bácsi, Attila ; To double acute zsér, József ; Benkő, Sz. / Ragweed pollen extract intensifies lipopolysaccharide-induced priming of NLRP3 inflammasome in human macrophages. In: Immunology. 2013 ; Vol. 138, No. 4. pp. 392-401.
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