Radioimmunoassays of tetrahydrocortisone and tetrahydrocortisol in human urine

H. Will, R. Aderjan, Th Winkler, B. Penke, P. Vecsei

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Abstract

A simple radioimmunoassay for the determination of tetrahydrocortisone (THE), and tetrahydrocortisol (THF) as well as their 21-glucosiduronate conjugates is described. White New Zealand rabbits were immunized with THF- and THR-20-oxime-bovine serum-albumin conjugates. The resulting antisera proved to be very specific. Both allo-THE and allo-THF gave minimal cross-reactions; however, THE- and THF-21-glucosiduronate bind with the corresponding antibodies at a level of 100%. The following types of estimations were introduced: a) estimations of the sum of unconjugated THF and THF-21-glucosiduronate in highly diluted unprocessed human urine. b) A similar estimation of THE and THF-21-glucosiduronate as in a). c) Estimation of THF after β-glucuronidase treatment and dilution without further procedures. d) A similar estimation for THE as in c). THF and THE values correlate significantly with known parameters that characterize the cortisol production, such as cortisol secretion rate and the urinary corticoid (C 21-α-ketolic-steroid) levels.

Original languageEnglish
Pages (from-to)369-379
Number of pages11
JournalActa endocrinologica
Volume86
Issue number2
Publication statusPublished - Dec 1 1977

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ASJC Scopus subject areas

  • Endocrinology

Cite this

Will, H., Aderjan, R., Winkler, T., Penke, B., & Vecsei, P. (1977). Radioimmunoassays of tetrahydrocortisone and tetrahydrocortisol in human urine. Acta endocrinologica, 86(2), 369-379.