Radioimmunoassay of CRF-Like Material in Rat Plasma: Validation of the Method

I. Torres-Aleman, S. Vigh, I. Merchenthaler, A. Somogyvari-Vigh, W. H. Carter, A. V. Schally

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The availability of antibodies against the ovine corticotropin releasing factor (CRF), which cross-react with a CRF-like immunoreactivity (CRF-LI) in the rat, has enabled us to develop a radioimmunoassay (RIA) for rat CRF-LI in plasma and crude hypothalamic extracts. 125I-Tyr CRF 1-41 was used as the tracer, and synthetic ovine CRF as the reference hormone. The precision profile of the assay indicates a high degree of reproducibility except for the lower dose range. The minimum detectable dose was 20 pg/tube. This assay can detect differences in plasma CRF-LI levels after various manipulations that simultaneously alter the ACTH levels in plasma. A wide range of CRF concentrations has been found in plasma of normal rats. Caution should be exercised in the interpretation of the values obtained since an ovine RIA system was used.

Original languageEnglish
Pages (from-to)355-361
Number of pages7
JournalProceedings of the Society for Experimental Biology and Medicine
Volume173
Issue number3
DOIs
Publication statusPublished - Jul 1983

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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