Radioimmunoassay of corticotropin from plasma

J. Gutkowska, J. Julesz, J. St-Louis, J. Genest

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We describe a specific and highly sensitive radioimmunoassay for corticotropin (ACTH) in human plasma. As little as 1.9 pg of circulating ACTH can be detected per milliliter (0.44 pmol/L). ACTH is first extracted from plasma samples by adsorption onto pretreated Vycor glass powder, eluted from the adsorbant by acetone, and then lyophilized. For purification of 125I-labeled ACTH, a small octadecasilyl silica column is used. The mean (and SD) concentration of ACTH in plasma from 18 ostensibly normal subjects was 10.3 (4.3) pmol/L. After overnight suppression with dexamethasone in seven of these normal subjects, their ACTH values fell to 2.62 (1.3) pmol/L (p <0.0005). This assay will permit clinical evaluation of ACTH plasma concentrations in clinical investigation and of the effects of drug administration on circulating ACTH.

Original languageEnglish
Pages (from-to)2229-2234
Number of pages6
JournalClinical Chemistry
Volume28
Issue number11
Publication statusPublished - 1982

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Adrenocorticotropic Hormone
Radioimmunoassay
Plasmas
Plasma (human)
Acetone
Powders
Dexamethasone
Adsorption
Purification
Glass
Assays
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Gutkowska, J., Julesz, J., St-Louis, J., & Genest, J. (1982). Radioimmunoassay of corticotropin from plasma. Clinical Chemistry, 28(11), 2229-2234.

Radioimmunoassay of corticotropin from plasma. / Gutkowska, J.; Julesz, J.; St-Louis, J.; Genest, J.

In: Clinical Chemistry, Vol. 28, No. 11, 1982, p. 2229-2234.

Research output: Contribution to journalArticle

Gutkowska, J, Julesz, J, St-Louis, J & Genest, J 1982, 'Radioimmunoassay of corticotropin from plasma', Clinical Chemistry, vol. 28, no. 11, pp. 2229-2234.
Gutkowska J, Julesz J, St-Louis J, Genest J. Radioimmunoassay of corticotropin from plasma. Clinical Chemistry. 1982;28(11):2229-2234.
Gutkowska, J. ; Julesz, J. ; St-Louis, J. ; Genest, J. / Radioimmunoassay of corticotropin from plasma. In: Clinical Chemistry. 1982 ; Vol. 28, No. 11. pp. 2229-2234.
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