Radioimmunoassay of bovine placental lactogen using recombinant and native preparations: Determination of fetal concentrations across gestation

A. V. Alvarez-Oxiley, N. M. Sousa, J. L. Hornick, K. Touati, G. C. Van Der Weijden, M. A.M. Taverne, O. Szenci, J. Sulon, P. Debliquy, J. F. Beckers

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Concentrations of bovine placental lactogen (bPL) were determined in fetal plasma samples by twelve double-antibody competitive radioimmunoassay systems (RIA I-XII) based on either recombinant bPL (non-glycosylated) or native bPL (glycosylated). Both preparations were used as standard and tracer, and for primary antisera production. The minimum detection limit measured by these RIA varied from 0.02 to 0.6 ng bPL mL-1. The coefficients of correlation of different bPL RIA systems were up to 90% (P < 0.0001) when each RIA was tested against the average values of all twelve RIA systems. All developed RIA were used to investigate the incidence of different bPL isoforms in bovine fetal serum samples (n = 71). Fetal concentrations ranged from 11.8 to 35.7 ng mL-1 at the third month and from 1.1 to 13.5 ng mL-1 at the ninth month of gestation. They tended to decrease with advancing gestation. In general, those RIA systems that used recombinant bPL as the standard measured higher values than those using the native bPL preparation. These differences decreased toward the end of gestation (P < 0.05), suggesting a lower rate of glycosylation. Our results provide evidence of different glycosylated isoforms of bPL in fetal serum at different gestation periods.

Original languageEnglish
Pages (from-to)877-885
Number of pages9
JournalReproduction, Fertility and Development
Volume19
Issue number7
DOIs
Publication statusPublished - Sep 11 2007

Keywords

  • Fetal serum
  • Glycosylated form

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

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    Alvarez-Oxiley, A. V., Sousa, N. M., Hornick, J. L., Touati, K., Van Der Weijden, G. C., Taverne, M. A. M., Szenci, O., Sulon, J., Debliquy, P., & Beckers, J. F. (2007). Radioimmunoassay of bovine placental lactogen using recombinant and native preparations: Determination of fetal concentrations across gestation. Reproduction, Fertility and Development, 19(7), 877-885. https://doi.org/10.1071/RD06173