Radioimmune double PEG precipitation technique for detecting complexed IgE

Katherine Meretey, Ute Böhm, A. Falus, S. Bozsoky

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Abstract

The presence of IgE in immune complex-enriched fractions prepared by 3% polyethylene glycol (PEG) precipitation was studied. The dissolved PEG-insoluble fraction was incubated with [125I]anti-IgE antibodies an then precipitated again with 3% PEG. Radioactivity in the washed pellets was taken as indicating IgE present in immune complex form. A calibration curve was constructed using artificially prepared IgE-anti-IgE complexes. The method developed may be useful to detect other components present in immune complex fractions.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalJournal of Immunological Methods
Volume26
Issue number3
DOIs
Publication statusPublished - Apr 10 1979

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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