The presence of IgE in immune complex-enriched fractions prepared by 3% polyethylene glycol (PEG) precipitation was studied. The dissolved PEG-insoluble fraction was incubated with [125I]anti-IgE antibodies an then precipitated again with 3% PEG. Radioactivity in the washed pellets was taken as indicating IgE present in immune complex form. A calibration curve was constructed using artificially prepared IgE-anti-IgE complexes. The method developed may be useful to detect other components present in immune complex fractions.
ASJC Scopus subject areas
- Immunology and Allergy