Radiodetoxified lipopolysaccharide fails to activate the hypophyseal-pituitary-adrenal axis in the rat

I. Barna, L. Bertók, J. I. Koenig, G. Makara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS) is known to raise the concentration of the circulating stress hormones such as ACTH, corticosterone and β-endorphin. This effect of endotoxin is mediated by different immune system-released hormone-like factors (e.g. interleukins, tumor necrosis factor etc.). Gamma-ray irradiation of LPS alters its biological properties and results in a radiodetoxified LPS preparation with numerous beneficial effects and decreased toxicity. In this study we compared the neuroendocrine effects of a commercial LPS and our native and radiode-toxified LPS preparations in rats. Plasma ACTH, corticosterone and β-endorphin levels were measured by specific radioimmunoassays 120 min after intraperitoneal LPS administration. Control animals were injected with saline. Results show a dramatic increase in all hormones after administration of commercial and our native LPS preparation. Hormone levels in saline-injected controls and radiodetoxified LPS-treated rats did not rise significantly. These results suggest that radio-detoxification disintegrated that part of the LPS molecule complex which is responsible for toxicity including an enhanced production of cytokines, which trigger the hypothalamo-pituitary-adrenal axis.

Original languageEnglish
Pages (from-to)128-131
Number of pages4
JournalNeuroImmunoModulation
Volume8
Issue number3
DOIs
Publication statusPublished - 2000

Fingerprint

Lipopolysaccharides
Hormones
Endorphins
Corticosterone
Adrenocorticotropic Hormone
Interleukins
Gamma Rays
Radio
Endotoxins
Radioimmunoassay
Immune System
Tumor Necrosis Factor-alpha
Cytokines

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Immunology

Cite this

Radiodetoxified lipopolysaccharide fails to activate the hypophyseal-pituitary-adrenal axis in the rat. / Barna, I.; Bertók, L.; Koenig, J. I.; Makara, G.

In: NeuroImmunoModulation, Vol. 8, No. 3, 2000, p. 128-131.

Research output: Contribution to journalArticle

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