The binding of radiochromate to tonsillar lymphocytes, the distribution of label, and the in vitro protein and nucleic acid synthesis of 51Cr labeled cells was investigated. The uptake of chromate by the cells is influenced by a variety of factors, namely, labeling time and concentration of chromate and of cells. The amount of chromate bound to a single cell increases with increasing cell concentration. The 51Cr labeling does not influence the in vitro protein and nucleic acid synthesis of the cells. It is shown that the distribution of label concerning the different cell fractions is determined by the chromium content of the cells. The labeling of the fraction insoluble in water and NP 40 is relatively high when the fraction is prepared from cells of high chromate content. Proteins produced in the course of in vitro incubation are also found to bind chromate. The results are discussed concerning the reliability and reproducibility of cytotoxicity assays and the interaction of chromate with lymphocytes.
|Number of pages||7|
|Journal||Journal of Nuclear Medicine|
|Publication status||Published - Dec 1 1974|
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging