Radiochromate binding capacity of human tonsillar lymphocytes

M. T. Szabo, A. Hrabák, F. Antoni

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The binding of radiochromate to tonsillar lymphocytes, the distribution of label, and the in vitro protein and nucleic acid synthesis of 51Cr labeled cells was investigated. The uptake of chromate by the cells is influenced by a variety of factors, namely, labeling time and concentration of chromate and of cells. The amount of chromate bound to a single cell increases with increasing cell concentration. The 51Cr labeling does not influence the in vitro protein and nucleic acid synthesis of the cells. It is shown that the distribution of label concerning the different cell fractions is determined by the chromium content of the cells. The labeling of the fraction insoluble in water and NP 40 is relatively high when the fraction is prepared from cells of high chromate content. Proteins produced in the course of in vitro incubation are also found to bind chromate. The results are discussed concerning the reliability and reproducibility of cytotoxicity assays and the interaction of chromate with lymphocytes.

Original languageEnglish
Pages (from-to)750-756
Number of pages7
JournalJournal of Nuclear Medicine
Volume15
Issue number9
Publication statusPublished - 1974

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Chromates
Lymphocytes
Nucleic Acids
Proteins
Chromium
Water
In Vitro Techniques

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Radiochromate binding capacity of human tonsillar lymphocytes. / Szabo, M. T.; Hrabák, A.; Antoni, F.

In: Journal of Nuclear Medicine, Vol. 15, No. 9, 1974, p. 750-756.

Research output: Contribution to journalArticle

Szabo, M. T. ; Hrabák, A. ; Antoni, F. / Radiochromate binding capacity of human tonsillar lymphocytes. In: Journal of Nuclear Medicine. 1974 ; Vol. 15, No. 9. pp. 750-756.
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