Radiobiological effects and proton RBE determined by wildtype zebrafish embryos

Emília Rita Szabó, Michael Brand, Stefan Hans, K. Hideghéty, Leonhard Karsch, Elisabeth Lessmann, Jörg Pawelke, Michael Schürer, Elke Beyreuther

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The increasing use of proton radiotherapy during the last decade and the rising number of long-term survivors has given rise to a vital discussion on potential effects on normal tissue. So far, deviations from clinically applied generic RBE (relative biological effectiveness) of 1.1 were only obtained by in vitro studies, whereas indications from in vivo trials and clinical studies are rare. In the present work, wildtype zebrafish embryos (Danio rerio) were used to characterize the effects of plateau and mid-SOBP (spread-out Bragg peak) proton radiation relative to that induced by clinical MV photon beam reference. Based on embryonic survival data, RBE values of 1.13 ± 0.08 and of 1.20 ± 0.04 were determined four days after irradiations with 20 Gy plateau and SOBP protons relative to 6 MV photon beams. These RBE values were confirmed by relating the rates of embryos with morphological abnormalities for the respective radiation qualities and doses. Besides survival, the rate of spine bending, as one type of developmental abnormality, and of pericardial edema, as an example for acute radiation effects, were assessed. The results revealed that independent on radiation quality both rates increased with time approaching almost 100% at the 4th day post irradiation with doses higher than 15 Gy. To sum up, the applicability of the zebrafish embryo as a robust and simple alternative model for in vivo characterization of radiobiological effects in normal tissue was validated and the obtained RBE values are comparable to previous finding in animal trials.

Original languageEnglish
Article numbere0206879
JournalPLoS One
Volume13
Issue number11
DOIs
Publication statusPublished - Nov 1 2018

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Relative Biological Effectiveness
Zebrafish
Danio rerio
protons
Protons
embryo (animal)
Embryonic Structures
Radiation
Dosimetry
plateaus
Photons
irradiation
Irradiation
Tissue
Radiation effects
abnormal development
Radiotherapy
radiotherapy
dosage
in vitro studies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Szabó, E. R., Brand, M., Hans, S., Hideghéty, K., Karsch, L., Lessmann, E., ... Beyreuther, E. (2018). Radiobiological effects and proton RBE determined by wildtype zebrafish embryos. PLoS One, 13(11), [e0206879]. https://doi.org/10.1371/journal.pone.0206879

Radiobiological effects and proton RBE determined by wildtype zebrafish embryos. / Szabó, Emília Rita; Brand, Michael; Hans, Stefan; Hideghéty, K.; Karsch, Leonhard; Lessmann, Elisabeth; Pawelke, Jörg; Schürer, Michael; Beyreuther, Elke.

In: PLoS One, Vol. 13, No. 11, e0206879, 01.11.2018.

Research output: Contribution to journalArticle

Szabó, ER, Brand, M, Hans, S, Hideghéty, K, Karsch, L, Lessmann, E, Pawelke, J, Schürer, M & Beyreuther, E 2018, 'Radiobiological effects and proton RBE determined by wildtype zebrafish embryos', PLoS One, vol. 13, no. 11, e0206879. https://doi.org/10.1371/journal.pone.0206879
Szabó, Emília Rita ; Brand, Michael ; Hans, Stefan ; Hideghéty, K. ; Karsch, Leonhard ; Lessmann, Elisabeth ; Pawelke, Jörg ; Schürer, Michael ; Beyreuther, Elke. / Radiobiological effects and proton RBE determined by wildtype zebrafish embryos. In: PLoS One. 2018 ; Vol. 13, No. 11.
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