Radiation-induced chromosomal rearrangement as an aid to analysis of the genetic constitution of Phaffia rhodozyma

Ágnes Nagy, Palágyi, Lajos Ferenczy, Csaba Vágvölgyi

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12 Citations (Scopus)

Abstract

Electrophoretic karyotypes of 80 auxotrophic and morphological mutants obtained from two Phaffia rhodozyma strains (ATCC 24203 and ATCC 24229) by γ-radiation were investigated. Contour-clamped homogeneous gel electrophoresis separation of the chromosomal size DNAs revealed 29 new chromosomal patterns after mutagen treatment. No correlation was found between a given type of chromosomal aberration and any phenotypic character. However, analysis of the chromosomal rearrangements proved to be useful for a more exact determination of chromosome number and genome size. The total genome size of ATCC 24229 was found to be 19.3 Mb, with nine chromosomes, while analysis of the mutant derivatives of ATCC 24203 suggested the presence of 11 chromosomes, with an estimated total genome size of 22.2 Mb. The advantages of the analysis of mutant electrophoretic karyotypes for genome characterization are discussed.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalFEMS microbiology letters
Volume152
Issue number2
DOIs
Publication statusPublished - Jul 15 1997

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Keywords

  • Chromosomal rearrangement
  • Contour-clamped homogeneous gel electrophoresis
  • Electrophoretic karyotype
  • Mutagenesis
  • Phaffia rhodozyma
  • γ- Irradiation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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