Radiation-induced changes of negative charge on the cell surface of primary human fibroblasts

Z. Somosy, Tamara Kubasova, G. S. Ecsedi, G. J. Köteles

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

By binding cationized ferritin (CF) to the plasma membrane of primary human fibroblasts, the amount and topology of negatively charged sites on cell surfaces were studied after X-irradiation. The CF binding was tested both on fixed and unfixed cells. Using various enzymes, the chemical nature of sites carrying the negative charges on cell surfaces was investigated. The results suggest that in unirradiated fibroblasts the CF binding occurred in a polarized manner, i.e. the particles were localized mainly on the apical surface of cells and formed clusters. The thin cytoplasmic protrusions and cell-to-cell contact sites bound CF to a greater extent than the bleb-like formations. Enzymatic digestion of surface polysaccharides showed that the main carriers of negatively charged sites are the glycosaminoglycans associated with the cell surface. The fixation of cells with glutaraldehyde did not influence the topology of CF binding either before or after the enzymatic treatment. After X-irradiation with 2·5 Gy the topology of CF binding did not change but the CF coverage of cells as well as the amount of ferritin particles per unit of suface area decreased within 10 min. The changes proved to be reversible as the values reached the pre-irradiation level by 1 h after irradiation.

Original languageEnglish
Pages (from-to)969-978
Number of pages10
JournalInternational Journal of Radiation Biology
Volume49
Issue number6
DOIs
Publication statusPublished - 1986

Fingerprint

fibroblasts
Fibroblasts
ferritin
Radiation
Irradiation
radiation
cells
Topology
irradiation
topology
Cell membranes
Polysaccharides
Enzymes
polysaccharides
enzymatic treatment
glutaraldehyde
glycosaminoglycans
Glutaral
Blister
Ferritins

Keywords

  • Cationized ferritin
  • Glycosidase
  • Human fibroblasts
  • Negative surface charges
  • X-irradiation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Radiation-induced changes of negative charge on the cell surface of primary human fibroblasts. / Somosy, Z.; Kubasova, Tamara; Ecsedi, G. S.; Köteles, G. J.

In: International Journal of Radiation Biology, Vol. 49, No. 6, 1986, p. 969-978.

Research output: Contribution to journalArticle

Somosy, Z. ; Kubasova, Tamara ; Ecsedi, G. S. ; Köteles, G. J. / Radiation-induced changes of negative charge on the cell surface of primary human fibroblasts. In: International Journal of Radiation Biology. 1986 ; Vol. 49, No. 6. pp. 969-978.
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