Rab2 promotes autophagic and endocytic lysosomal degradation

Péter Lörincz, Sarolta Tóth, Péter Benkö, Zsolt Lakatos, Attila Boda, Gábor Glatz, Martina Zobel, Sara Bisi, Krisztina Hegedüs, Szabolcs Takáts, Giorgio Scita, G. Juhász

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Rab7 promotes fusion of autophagosomes and late endosomes with lysosomes in yeast and metazoan cells, acting together with its effector, the tethering complex HOPS. Here we show that another small GTPase, Rab2, is also required for autophagosome and endosome maturation and proper lysosome function in Drosophila melanogaster. We demonstrate that Rab2 binds to HOPS, and that its active, GTP-locked form associates with autolysosomes. Importantly, expression of active Rab2 promotes autolysosomal fusions unlike that of GTP-locked Rab7, suggesting that its amount is normally rate limiting. We also demonstrate that RAB2A is required for autophagosome clearance in human breast cancer cells. In conclusion, we identify Rab2 as a key factor for autophagic and endocytic cargo delivery to and degradation in lysosomes.

Original languageEnglish
Pages (from-to)1937-1947
Number of pages11
JournalJournal of Cell Biology
Volume216
Issue number7
DOIs
Publication statusPublished - Jul 1 2017

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Lysosomes
Endosomes
Guanosine Triphosphate
Monomeric GTP-Binding Proteins
Drosophila melanogaster
Yeasts
Breast Neoplasms
Autophagosomes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lörincz, P., Tóth, S., Benkö, P., Lakatos, Z., Boda, A., Glatz, G., ... Juhász, G. (2017). Rab2 promotes autophagic and endocytic lysosomal degradation. Journal of Cell Biology, 216(7), 1937-1947. https://doi.org/10.1083/jcb.201611027

Rab2 promotes autophagic and endocytic lysosomal degradation. / Lörincz, Péter; Tóth, Sarolta; Benkö, Péter; Lakatos, Zsolt; Boda, Attila; Glatz, Gábor; Zobel, Martina; Bisi, Sara; Hegedüs, Krisztina; Takáts, Szabolcs; Scita, Giorgio; Juhász, G.

In: Journal of Cell Biology, Vol. 216, No. 7, 01.07.2017, p. 1937-1947.

Research output: Contribution to journalArticle

Lörincz, P, Tóth, S, Benkö, P, Lakatos, Z, Boda, A, Glatz, G, Zobel, M, Bisi, S, Hegedüs, K, Takáts, S, Scita, G & Juhász, G 2017, 'Rab2 promotes autophagic and endocytic lysosomal degradation', Journal of Cell Biology, vol. 216, no. 7, pp. 1937-1947. https://doi.org/10.1083/jcb.201611027
Lörincz P, Tóth S, Benkö P, Lakatos Z, Boda A, Glatz G et al. Rab2 promotes autophagic and endocytic lysosomal degradation. Journal of Cell Biology. 2017 Jul 1;216(7):1937-1947. https://doi.org/10.1083/jcb.201611027
Lörincz, Péter ; Tóth, Sarolta ; Benkö, Péter ; Lakatos, Zsolt ; Boda, Attila ; Glatz, Gábor ; Zobel, Martina ; Bisi, Sara ; Hegedüs, Krisztina ; Takáts, Szabolcs ; Scita, Giorgio ; Juhász, G. / Rab2 promotes autophagic and endocytic lysosomal degradation. In: Journal of Cell Biology. 2017 ; Vol. 216, No. 7. pp. 1937-1947.
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