Rab11 facilitates cross-talk between autophagy and endosomal pathway through regulation of Hook localization

Zsuzsanna Szatmári, Viktor Kis, Mónika Lippai, Krisztina Hegedus, Tamás Faragó, Péter Lorincz, Tsubasa Tanaka, G. Juhász, M. Sass

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

During autophagy, double-membrane autophagosomes deliver sequestered cytoplasmic content to late endosomes and lysosomes for degradation. The molecular mechanism of autophagosome maturation is still poorly characterized. The small GTPase Rab11 regulates endosomal traffic and is thought to function at the level of recycling endosomes. We show that loss of Rab11 leads to accumulation of autophagosomes and late endosomes in Drosophila melanogaster. Rab11 translocates from recycling endosomes to autophagosomes in response to autophagy induction and physically interacts with Hook, a negative regulator of endosome maturation. Hook anchors endosomes to microtubules, and we show that Rab11 facilitates the fusion of endosomes and autophagosomes by removing Hook from mature late endosomes and inhibiting its homodimerization. Thus induction of autophagy appears to promote autophagic flux by increased convergence with the endosomal pathway.

Original languageEnglish
Pages (from-to)522-531
Number of pages10
JournalMolecular Biology of the Cell
Volume25
Issue number4
DOIs
Publication statusPublished - Feb 15 2014

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Endosomes
Autophagy
Monomeric GTP-Binding Proteins
Lysosomes
Drosophila melanogaster
Microtubules
Autophagosomes
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

Rab11 facilitates cross-talk between autophagy and endosomal pathway through regulation of Hook localization. / Szatmári, Zsuzsanna; Kis, Viktor; Lippai, Mónika; Hegedus, Krisztina; Faragó, Tamás; Lorincz, Péter; Tanaka, Tsubasa; Juhász, G.; Sass, M.

In: Molecular Biology of the Cell, Vol. 25, No. 4, 15.02.2014, p. 522-531.

Research output: Contribution to journalArticle

Szatmári, Zsuzsanna ; Kis, Viktor ; Lippai, Mónika ; Hegedus, Krisztina ; Faragó, Tamás ; Lorincz, Péter ; Tanaka, Tsubasa ; Juhász, G. ; Sass, M. / Rab11 facilitates cross-talk between autophagy and endosomal pathway through regulation of Hook localization. In: Molecular Biology of the Cell. 2014 ; Vol. 25, No. 4. pp. 522-531.
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