Quorum-sensing signal production by Agrobacterium vitis strains and their tumor-inducing and tartrate-catabolic plasmids

Nathanial Lowe, Han Ming Gan, Vandana Chakravartty, Russell Scott, E. Szegedi, Thomas J. Burr, Michael A. Savka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Agrobacterium vitis strains, their tumor-inducing (pTi) and tartrate utilization (pTr) plasmid transconjugants and grapevine tumors were analyzed for the presence of N-acyl-homoserine lactones (AHLs). All wild-type A. vitis strains produced long-chain signals. PCR analysis of the A. vitis long-chain AHL synthase gene, avsI, showed the predicted amplicon. Agrobacterium tumefaciens UBAPF2 harboring various A. vitis pTi plasmids produced N-(3-oxo-octanoyl)-l- homoserine lactone encoded also by pTis of A. tumefaciens. UBAPF2 transconjugants carrying pTrs except for pTrTm4 and pTrAB3, also produced an AHL. UBAPF2 transconjugants carrying pTrAT6, pTrAB4 and pTrRr4 or pTiNi1 produced two additional AHLs not observed in the corresponding wild-type strains. We also provide evidence for in situ production of AHLs in grapevine crown gall tumors of greenhouse and field origin.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalFEMS Microbiology Letters
Volume296
Issue number1
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Acyl-Butyrolactones
Quorum Sensing
Agrobacterium
Vitis
Plasmids
Agrobacterium tumefaciens
Neoplasms
Plant Tumors
tartaric acid
Polymerase Chain Reaction
Genes

Keywords

  • Acyl-homoserine lactone
  • Crown gall
  • Grapevine
  • Opines
  • Vitis

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Quorum-sensing signal production by Agrobacterium vitis strains and their tumor-inducing and tartrate-catabolic plasmids. / Lowe, Nathanial; Gan, Han Ming; Chakravartty, Vandana; Scott, Russell; Szegedi, E.; Burr, Thomas J.; Savka, Michael A.

In: FEMS Microbiology Letters, Vol. 296, No. 1, 07.2009, p. 102-109.

Research output: Contribution to journalArticle

Lowe, Nathanial ; Gan, Han Ming ; Chakravartty, Vandana ; Scott, Russell ; Szegedi, E. ; Burr, Thomas J. ; Savka, Michael A. / Quorum-sensing signal production by Agrobacterium vitis strains and their tumor-inducing and tartrate-catabolic plasmids. In: FEMS Microbiology Letters. 2009 ; Vol. 296, No. 1. pp. 102-109.
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