Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis

S. Nagy, Péter J. Polgár, Magnus Andersson, A. Kovács

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to test the FXCycle PI/RNase kit for routine DNA analyses in order to detect breeding bulls and/or insemination doses carrying cytogenetic aberrations. In a series of experiments we first established basic DNA histogram parameters of cytogenetically healthy breeding bulls by measuring the intraspecific genome size variation of three animals, then we compared the histogram profiles of bulls carrying cytogenetic defects to the baseline values. With the exception of one case the test was able to identify bulls with cytogenetic defects. Therefore, we conclude that the assay could be incorporated into the laboratory routine where flow cytometry is applied for semen quality control.

Original languageEnglish
Pages (from-to)372-379
Number of pages8
JournalActa Veterinaria Hungarica
Volume64
Issue number3
DOIs
Publication statusPublished - Sep 1 2016

Keywords

  • Cytogenetics
  • Flow cytometry
  • Sperm
  • Translocation

ASJC Scopus subject areas

  • veterinary(all)

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