A vérplazma összes szabad DNS, valamint szabad magzati DNS mennyisége praeeclampsiával szövodött és szövodménymentes terhességek esetében

Translated title of the contribution: Quantity of total cell free and cell free fetal DNA in pregnancies with no complications and with preeclampsia

Levente Lázár, B. Nagy, A. Molvarec, J. Rigó

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Several researches focused on the factors which could influence the quantity of cell free DNA in case of normal and pathological pregnancies. The aim of our study was to evaluate the quantity of total cell free and cell free fetal DNA in case of normal pregnancies and preeclampsia. Study design: Plasma samples were obtained from 67 preeclamptic and 70 normotensive pregnant women. The quantity of total cell free DNA and cell free fetal DNA was measured using real-time polymerase chain reaction. Results: We confirmed that circulating total free and fetal DNA levels are significantly elevated in pregnancies complicated by preeclampsia (median: 0.0114 vs. 0.0325 and 0.001E-3 vs. 0.086E-3 ng/μl; P <0.001). The quantity of total plasma-free DNA did not correlate with the body mass index. Conclusion: The releases of both free fetal and maternal DNA were found to be affected in preeclampsia. Hepatocellular necrosis seems to be responsible-at least partly-for increased circulating total DNA levels in preeclampsia, and the abnormal trophoblast invasion could be responsible for increased trophoblast destruction and elevation of cell free fetal DNA level.

Original languageHungarian
Pages (from-to)784-787
Number of pages4
JournalOrvosi Hetilap
Volume151
Issue number19
DOIs
Publication statusPublished - May 1 2010

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Pre-Eclampsia
Pregnancy
DNA
Trophoblasts
Real-Time Polymerase Chain Reaction
Pregnant Women
Body Mass Index
Necrosis
Mothers
Research

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "A v{\'e}rplazma {\"o}sszes szabad DNS, valamint szabad magzati DNS mennyis{\'e}ge praeeclampsi{\'a}val sz{\"o}vod{\"o}tt {\'e}s sz{\"o}vodm{\'e}nymentes terhess{\'e}gek eset{\'e}ben",
abstract = "Several researches focused on the factors which could influence the quantity of cell free DNA in case of normal and pathological pregnancies. The aim of our study was to evaluate the quantity of total cell free and cell free fetal DNA in case of normal pregnancies and preeclampsia. Study design: Plasma samples were obtained from 67 preeclamptic and 70 normotensive pregnant women. The quantity of total cell free DNA and cell free fetal DNA was measured using real-time polymerase chain reaction. Results: We confirmed that circulating total free and fetal DNA levels are significantly elevated in pregnancies complicated by preeclampsia (median: 0.0114 vs. 0.0325 and 0.001E-3 vs. 0.086E-3 ng/μl; P <0.001). The quantity of total plasma-free DNA did not correlate with the body mass index. Conclusion: The releases of both free fetal and maternal DNA were found to be affected in preeclampsia. Hepatocellular necrosis seems to be responsible-at least partly-for increased circulating total DNA levels in preeclampsia, and the abnormal trophoblast invasion could be responsible for increased trophoblast destruction and elevation of cell free fetal DNA level.",
keywords = "Body mass index, Cell free fetal DNA, Preeclampsia, Total cell free DNA",
author = "Levente L{\'a}z{\'a}r and B. Nagy and A. Molvarec and J. Rig{\'o}",
year = "2010",
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AU - Nagy, B.

AU - Molvarec, A.

AU - Rigó, J.

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N2 - Several researches focused on the factors which could influence the quantity of cell free DNA in case of normal and pathological pregnancies. The aim of our study was to evaluate the quantity of total cell free and cell free fetal DNA in case of normal pregnancies and preeclampsia. Study design: Plasma samples were obtained from 67 preeclamptic and 70 normotensive pregnant women. The quantity of total cell free DNA and cell free fetal DNA was measured using real-time polymerase chain reaction. Results: We confirmed that circulating total free and fetal DNA levels are significantly elevated in pregnancies complicated by preeclampsia (median: 0.0114 vs. 0.0325 and 0.001E-3 vs. 0.086E-3 ng/μl; P <0.001). The quantity of total plasma-free DNA did not correlate with the body mass index. Conclusion: The releases of both free fetal and maternal DNA were found to be affected in preeclampsia. Hepatocellular necrosis seems to be responsible-at least partly-for increased circulating total DNA levels in preeclampsia, and the abnormal trophoblast invasion could be responsible for increased trophoblast destruction and elevation of cell free fetal DNA level.

AB - Several researches focused on the factors which could influence the quantity of cell free DNA in case of normal and pathological pregnancies. The aim of our study was to evaluate the quantity of total cell free and cell free fetal DNA in case of normal pregnancies and preeclampsia. Study design: Plasma samples were obtained from 67 preeclamptic and 70 normotensive pregnant women. The quantity of total cell free DNA and cell free fetal DNA was measured using real-time polymerase chain reaction. Results: We confirmed that circulating total free and fetal DNA levels are significantly elevated in pregnancies complicated by preeclampsia (median: 0.0114 vs. 0.0325 and 0.001E-3 vs. 0.086E-3 ng/μl; P <0.001). The quantity of total plasma-free DNA did not correlate with the body mass index. Conclusion: The releases of both free fetal and maternal DNA were found to be affected in preeclampsia. Hepatocellular necrosis seems to be responsible-at least partly-for increased circulating total DNA levels in preeclampsia, and the abnormal trophoblast invasion could be responsible for increased trophoblast destruction and elevation of cell free fetal DNA level.

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KW - Total cell free DNA

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