Quantitative test of physical and chemical effects on microbiological models of chromosomes, cells and viruses using physical measurements

G. Rontó, I. Derka, S. Gaspar, K. Módos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Certain damaging effects of chemical agents and UV light on bacteriophage, bacterium and phage-bacterium complexes can be quantitatively evaluated by physical measurements. A novel method is described for the quantitative characterization of the genotoxic effect of chemicals. The method, which is based on the inactivation kinetics of bacteriophage, seems ideal for modeling of the DNA damage process. E. coli B bacterium is infected by the chemical-treated phage, and the resulting parameters (multiplicity of infection, burst time, and its deviation) of the first phage developmental cycle can be measured optically. Knowing the dose of the treatment, the characteristic quantity of the phage inactivation is obtainable. The mutagenicity index (MI) is introduced to characterize the genotoxic effect quantitatively. The method and measurement apparatus described provide automatic testing of large series of chemicals and determining their mutagenicity index and their cellular and viral toxicity.

Original languageEnglish
Title of host publicationIEEE/Engineering in Medicine and Biology Society Annual Conference
PublisherPubl by IEEE
Pages1027-1028
Number of pages2
Volume10
Editionpt2
Publication statusPublished - Nov 1988
EventProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society - New Orleans, LA, USA
Duration: Nov 4 1988Nov 7 1988

Other

OtherProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CityNew Orleans, LA, USA
Period11/4/8811/7/88

Fingerprint

Bacteriophages
Chromosomes
Viruses
Bacteria
Automatic testing
Ultraviolet radiation
Escherichia coli
Toxicity
DNA
Kinetics

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Rontó, G., Derka, I., Gaspar, S., & Módos, K. (1988). Quantitative test of physical and chemical effects on microbiological models of chromosomes, cells and viruses using physical measurements. In IEEE/Engineering in Medicine and Biology Society Annual Conference (pt2 ed., Vol. 10, pp. 1027-1028). Publ by IEEE.

Quantitative test of physical and chemical effects on microbiological models of chromosomes, cells and viruses using physical measurements. / Rontó, G.; Derka, I.; Gaspar, S.; Módos, K.

IEEE/Engineering in Medicine and Biology Society Annual Conference. Vol. 10 pt2. ed. Publ by IEEE, 1988. p. 1027-1028.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rontó, G, Derka, I, Gaspar, S & Módos, K 1988, Quantitative test of physical and chemical effects on microbiological models of chromosomes, cells and viruses using physical measurements. in IEEE/Engineering in Medicine and Biology Society Annual Conference. pt2 edn, vol. 10, Publ by IEEE, pp. 1027-1028, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, New Orleans, LA, USA, 11/4/88.
Rontó G, Derka I, Gaspar S, Módos K. Quantitative test of physical and chemical effects on microbiological models of chromosomes, cells and viruses using physical measurements. In IEEE/Engineering in Medicine and Biology Society Annual Conference. pt2 ed. Vol. 10. Publ by IEEE. 1988. p. 1027-1028
Rontó, G. ; Derka, I. ; Gaspar, S. ; Módos, K. / Quantitative test of physical and chemical effects on microbiological models of chromosomes, cells and viruses using physical measurements. IEEE/Engineering in Medicine and Biology Society Annual Conference. Vol. 10 pt2. ed. Publ by IEEE, 1988. pp. 1027-1028
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