Quantitative test of physical and chemical effects on microbiological models of chromosomes, cells and viruses using physical measurements

Gy Ronto, I. Derka, S. Gaspar, K. Modos

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

Certain damaging effects of chemical agents and UV light on bacteriophage, bacterium and phage-bacterium complexes can be quantitatively evaluated by physical measurements. A novel method is described for the quantitative characterization of the genotoxic effect of chemicals. The method, which is based on the inactivation kinetics of bacteriophage, seems ideal for modeling of the DNA damage process. E. coli B bacterium is infected by the chemical-treated phage, and the resulting parameters (multiplicity of infection, burst time, and its deviation) of the first phage developmental cycle can be measured optically. Knowing the dose of the treatment, the characteristic quantity of the phage inactivation is obtainable. The mutagenicity index (MI) is introduced to characterize the genotoxic effect quantitatively. The method and measurement apparatus described provide automatic testing of large series of chemicals and determining their mutagenicity index and their cellular and viral toxicity.

Original languageEnglish
Pages1027-1028
Number of pages2
Publication statusPublished - Nov 1 1988
EventProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society - New Orleans, LA, USA
Duration: Nov 4 1988Nov 7 1988

Other

OtherProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CityNew Orleans, LA, USA
Period11/4/8811/7/88

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ASJC Scopus subject areas

  • Engineering(all)

Cite this

Ronto, G., Derka, I., Gaspar, S., & Modos, K. (1988). Quantitative test of physical and chemical effects on microbiological models of chromosomes, cells and viruses using physical measurements. 1027-1028. Paper presented at Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, New Orleans, LA, USA, .