Quantitative relationship of catecholamine content and histofluorescence in brain of rats

Irwin J. Kopin, Palkovits Miklos, Ronald M. Kobayashi, David M. Jacobowitz

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Levels of catecholamine content assayed by a sensitive radiometric technique were compared to semi-quantitative visual estimation of the histofluorescence in different regions of brain after amine depletion by varying doses of reserpine. The range over which fluorescence of varicose terminals appeared to diminish is only about 20-40% of the normal catecholamine content. The level of this range varied with the area examined. The percent of normal catecholamine levels at which fluorescence was first noticed to be decreased from normal, and that at which fluorescence seemed to disappear were relatively close and both appeared to be linearly correlated to the logarithm of the normal catecholamine content. In areas with a normally high catecholamine content, a greater percentage decrease in amine content was required to make a noticeable decrease in fluorescence than was required in areas with a normally lower catecholamine content.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalBrain Research
Volume80
Issue number2
DOIs
Publication statusPublished - Nov 15 1974

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Catecholamines
Brain
Fluorescence
Amines
Reserpine

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Quantitative relationship of catecholamine content and histofluorescence in brain of rats. / Kopin, Irwin J.; Miklos, Palkovits; Kobayashi, Ronald M.; Jacobowitz, David M.

In: Brain Research, Vol. 80, No. 2, 15.11.1974, p. 229-235.

Research output: Contribution to journalArticle

Kopin, Irwin J. ; Miklos, Palkovits ; Kobayashi, Ronald M. ; Jacobowitz, David M. / Quantitative relationship of catecholamine content and histofluorescence in brain of rats. In: Brain Research. 1974 ; Vol. 80, No. 2. pp. 229-235.
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