Quantitative phenotypic and functional analyses of islet immune cells before and after diabetes onset in the BB rat

N. Hosszúfalusi, E. Chan, M. Teruya, S. Takei, G. Granger, M. A. Charles

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Inflammatory cells invading islets are thought to be mediators of islet destruction in spontaneous autoimmune diabetes mellitus. Thus methods were developed to isolate and characterize in situ islet inflammatory cells from 75-95-day-old prediabetic and diabetic BB rats. Islet inflammatory cells were structurally examined using single- and double-colour flow cytometry. Functional studies consisted of cytolytic assays using normal rat islet target cells and in situ islet or spleen effector cells. Structural data reveal natural killer cells to be the major cell population (70%) of total immune cells present in inflamed islets during prediabetes. At diabetes onset, the natural killer cell population remained at a high level (47%), but an increasing population of T cells (40%) was noted also. Analyses of T-cell subsets before and after diabetes onset revealed CD4+ T cells as predominant (50-55% of total T cells) with double-negative (CD4- CD8-) T cells (25-30%) and CD8+ T cells (15-20%) also present in significant quantities. Activated T cells accounted only for a minority of T cells (

Original languageEnglish
Pages (from-to)1146-1154
Number of pages9
JournalDiabetologia
Volume36
Issue number11
DOIs
Publication statusPublished - Nov 1993

Fingerprint

Inbred BB Rats
Islets of Langerhans
T-Lymphocytes
Natural Killer Cells
Population
Prediabetic State
T-Lymphocyte Subsets
Type 1 Diabetes Mellitus
Diabetes Mellitus
Flow Cytometry
Spleen
Color

Keywords

  • BB rat
  • diabetes mellitus
  • in situ islet immune cells
  • inflamed islets
  • islets

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Quantitative phenotypic and functional analyses of islet immune cells before and after diabetes onset in the BB rat. / Hosszúfalusi, N.; Chan, E.; Teruya, M.; Takei, S.; Granger, G.; Charles, M. A.

In: Diabetologia, Vol. 36, No. 11, 11.1993, p. 1146-1154.

Research output: Contribution to journalArticle

Hosszúfalusi, N. ; Chan, E. ; Teruya, M. ; Takei, S. ; Granger, G. ; Charles, M. A. / Quantitative phenotypic and functional analyses of islet immune cells before and after diabetes onset in the BB rat. In: Diabetologia. 1993 ; Vol. 36, No. 11. pp. 1146-1154.
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