Quantitative methods for studying the role of free radicals in biology

J. Jakus, Tamas Kriska, Tatjana K. Maximova, Andras Nemeth, L. Korecz, Dezso Gal

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The mechanism of the primary sensitization steps in Photodynamic Therapy could be of essential importance concerning the efficiency of the treatment. Besides the well known mechanisms there is a possibility that the interaction between the excited triplet state sensitizer and the doublet state free radicals generated by the cells adds a significant contribution to the overall photodynamic effect. To support this hypothesis the quantification and identification of free radicals would be required. For this purpose chemiluminescence studies and spin trapping seemed to be the most suitable methods due to the extremely short lifetime of free radicals in biological systems. We present data on the kinetics of accumulation of free radicals: by measuring luminol-dependent chemiluminescence of stimulated macrophages both in the absence and in the presence of a free radical inhibitor 3,5 di-tert-butyl-4-hydroxyphenyl propionic acid; by following the production of primary, secondary and tertiary radical adducts using DMPO (5,5-dimethyl-1-pyrroline N-oxide) as spin trapping agent in chemical model systems. The measured data are used to determine the effect of the excited sensitizers on the kinetics of accumulation of free radicals under biological conditions. These results would serve to design new and more effective sensitizers used for therapy based on the triplet-doublet mechanism of action.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages100-111
Number of pages12
Volume3563
Publication statusPublished - 1999
EventProceedings of the 1998 Photochemotherapy of Cancer and Other Diseases - Stockholm, SWE
Duration: Sep 9 1998Sep 11 1998

Other

OtherProceedings of the 1998 Photochemotherapy of Cancer and Other Diseases
CityStockholm, SWE
Period9/9/989/11/98

Fingerprint

Free radicals
biology
free radicals
Chemiluminescence
chemiluminescence
therapy
trapping
propionic acid
Propionic acid
Photodynamic therapy
Kinetics
macrophages
Macrophages
kinetics
Biological systems
Excited states
inhibitors
atomic energy levels
adducts
life (durability)

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Jakus, J., Kriska, T., Maximova, T. K., Nemeth, A., Korecz, L., & Gal, D. (1999). Quantitative methods for studying the role of free radicals in biology. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3563, pp. 100-111). Society of Photo-Optical Instrumentation Engineers.

Quantitative methods for studying the role of free radicals in biology. / Jakus, J.; Kriska, Tamas; Maximova, Tatjana K.; Nemeth, Andras; Korecz, L.; Gal, Dezso.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3563 Society of Photo-Optical Instrumentation Engineers, 1999. p. 100-111.

Research output: Chapter in Book/Report/Conference proceedingChapter

Jakus, J, Kriska, T, Maximova, TK, Nemeth, A, Korecz, L & Gal, D 1999, Quantitative methods for studying the role of free radicals in biology. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3563, Society of Photo-Optical Instrumentation Engineers, pp. 100-111, Proceedings of the 1998 Photochemotherapy of Cancer and Other Diseases, Stockholm, SWE, 9/9/98.
Jakus J, Kriska T, Maximova TK, Nemeth A, Korecz L, Gal D. Quantitative methods for studying the role of free radicals in biology. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3563. Society of Photo-Optical Instrumentation Engineers. 1999. p. 100-111
Jakus, J. ; Kriska, Tamas ; Maximova, Tatjana K. ; Nemeth, Andras ; Korecz, L. ; Gal, Dezso. / Quantitative methods for studying the role of free radicals in biology. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3563 Society of Photo-Optical Instrumentation Engineers, 1999. pp. 100-111
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