Quantitative LA-ICP-MS imaging of platinum in chemotherapy treated human malignant pleural mesothelioma samples using printed patterns as standard

Maximilian Bonta, Hans Lohninger, Viktoria Laszlo, B. Hegedűs, Andreas Limbeck

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

LA-ICP-MS has often been applied for the analysis of trace elemental distributions in biological tissues. However, the strong matrix dependence of LA-ICP-MS analyses and highly variable matrix conditions aggravate reliable qualitative distribution analyses and thus for obtaining quantitative information elaborate quantification strategies have to be applied. In this work printed patterns on paper with thin gold layers as pseudo-internal standard have been proposed as an alternative approach to the commonly used matrix-matched tissue standards. Besides a major reduction of the workload for standard preparation the presented method allows for compensation of instrumental drifts during measurement as well as a reduction of matrix related effects. The developed method has been verified using matrix-matched tissue standards (deviations from the actual metal content less than 5% with relative standard deviations of less than 7%) and applied to platinum imaging on human malignant pleural mesothelioma samples after administration of individuals with platinum containing cytostatic drugs.

Original languageEnglish
Pages (from-to)2159-2167
Number of pages9
JournalJournal of Analytical Atomic Spectrometry
Volume29
Issue number11
DOIs
Publication statusPublished - Nov 1 2014

Fingerprint

Chemotherapy
Platinum
Imaging techniques
Tissue
Cytostatic Agents
Gold
Metals

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Quantitative LA-ICP-MS imaging of platinum in chemotherapy treated human malignant pleural mesothelioma samples using printed patterns as standard. / Bonta, Maximilian; Lohninger, Hans; Laszlo, Viktoria; Hegedűs, B.; Limbeck, Andreas.

In: Journal of Analytical Atomic Spectrometry, Vol. 29, No. 11, 01.11.2014, p. 2159-2167.

Research output: Contribution to journalArticle

@article{f42f051101f94b27806668d7cc3f20f8,
title = "Quantitative LA-ICP-MS imaging of platinum in chemotherapy treated human malignant pleural mesothelioma samples using printed patterns as standard",
abstract = "LA-ICP-MS has often been applied for the analysis of trace elemental distributions in biological tissues. However, the strong matrix dependence of LA-ICP-MS analyses and highly variable matrix conditions aggravate reliable qualitative distribution analyses and thus for obtaining quantitative information elaborate quantification strategies have to be applied. In this work printed patterns on paper with thin gold layers as pseudo-internal standard have been proposed as an alternative approach to the commonly used matrix-matched tissue standards. Besides a major reduction of the workload for standard preparation the presented method allows for compensation of instrumental drifts during measurement as well as a reduction of matrix related effects. The developed method has been verified using matrix-matched tissue standards (deviations from the actual metal content less than 5{\%} with relative standard deviations of less than 7{\%}) and applied to platinum imaging on human malignant pleural mesothelioma samples after administration of individuals with platinum containing cytostatic drugs.",
author = "Maximilian Bonta and Hans Lohninger and Viktoria Laszlo and B. Hegedűs and Andreas Limbeck",
year = "2014",
month = "11",
day = "1",
doi = "10.1039/c4ja00245h",
language = "English",
volume = "29",
pages = "2159--2167",
journal = "Journal of Analytical Atomic Spectrometry",
issn = "0267-9477",
publisher = "Royal Society of Chemistry",
number = "11",

}

TY - JOUR

T1 - Quantitative LA-ICP-MS imaging of platinum in chemotherapy treated human malignant pleural mesothelioma samples using printed patterns as standard

AU - Bonta, Maximilian

AU - Lohninger, Hans

AU - Laszlo, Viktoria

AU - Hegedűs, B.

AU - Limbeck, Andreas

PY - 2014/11/1

Y1 - 2014/11/1

N2 - LA-ICP-MS has often been applied for the analysis of trace elemental distributions in biological tissues. However, the strong matrix dependence of LA-ICP-MS analyses and highly variable matrix conditions aggravate reliable qualitative distribution analyses and thus for obtaining quantitative information elaborate quantification strategies have to be applied. In this work printed patterns on paper with thin gold layers as pseudo-internal standard have been proposed as an alternative approach to the commonly used matrix-matched tissue standards. Besides a major reduction of the workload for standard preparation the presented method allows for compensation of instrumental drifts during measurement as well as a reduction of matrix related effects. The developed method has been verified using matrix-matched tissue standards (deviations from the actual metal content less than 5% with relative standard deviations of less than 7%) and applied to platinum imaging on human malignant pleural mesothelioma samples after administration of individuals with platinum containing cytostatic drugs.

AB - LA-ICP-MS has often been applied for the analysis of trace elemental distributions in biological tissues. However, the strong matrix dependence of LA-ICP-MS analyses and highly variable matrix conditions aggravate reliable qualitative distribution analyses and thus for obtaining quantitative information elaborate quantification strategies have to be applied. In this work printed patterns on paper with thin gold layers as pseudo-internal standard have been proposed as an alternative approach to the commonly used matrix-matched tissue standards. Besides a major reduction of the workload for standard preparation the presented method allows for compensation of instrumental drifts during measurement as well as a reduction of matrix related effects. The developed method has been verified using matrix-matched tissue standards (deviations from the actual metal content less than 5% with relative standard deviations of less than 7%) and applied to platinum imaging on human malignant pleural mesothelioma samples after administration of individuals with platinum containing cytostatic drugs.

UR - http://www.scopus.com/inward/record.url?scp=84907997436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907997436&partnerID=8YFLogxK

U2 - 10.1039/c4ja00245h

DO - 10.1039/c4ja00245h

M3 - Article

AN - SCOPUS:84907997436

VL - 29

SP - 2159

EP - 2167

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

IS - 11

ER -