Quantitative determination of hyaluronan content in cerebral aneurysms by digital densitometry

A. Klekner, S. Felszeghy, R. Tammi, M. Tammi, G. Csécsei, L. Módis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Object: Hyaluronan (HA) is a highly hydrated macromolecule; it is one of the essential components of the extracellular matrix (ECM) of the arteries and plays an important role in maintaining the biomechanical features of blood vessels. Although the potential contribution of HA in aneurysms of different vessels has been studied intensively, no data are available about the alteration of the HA content in the extracellular matrix of intracranial aneurysms. The aim of the study was to determine the hyaluronan content in the wall of human cerebral arteries. Methods: A biotinylated aggrecan fragment that binds specifically to HA was used to stain samples from cerebral aneurysms (n = 11) to compare the HA content to non-aneurysmal arteries of patients who had intracranial aneurysm (n = 11), and to histologically normal arteries of patients who had expired from non-vascular diseases (n = 14). Digital microscopic densitometry was used for the quantitative analysis of the hyaluronan content in these samples. Results: The highest level (169.5 ±7.9) was detected in aneurysms, while the HA-level of non-aneurysmal vessels was lower (130.2 ± 16.8). Both vessel groups contained significantly higher HA than the normal cerebral arteries (32.9 ± 2.1). Conclusions: Results suggest that an elevated hyaluronan level in the extracellular matrix may affect the cerebral arterial wall architecture. It is reasonable to suppose that the increased hyaluronan content creates a viscoelastic ECM which might improve the biomechanical resistance of the thinned vessel wall.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalZentralblatt fur Neurochirurgie
Volume66
Issue number4
DOIs
Publication statusPublished - 2005

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Densitometry
Intracranial Aneurysm
Hyaluronic Acid
Extracellular Matrix
Cerebral Arteries
Arteries
Aneurysm
Aggrecans
Blood Vessels
Coloring Agents

Keywords

  • Cerebral aneurysm
  • Digital densitometry
  • Extracellular matrix
  • Hyaluronan

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Quantitative determination of hyaluronan content in cerebral aneurysms by digital densitometry. / Klekner, A.; Felszeghy, S.; Tammi, R.; Tammi, M.; Csécsei, G.; Módis, L.

In: Zentralblatt fur Neurochirurgie, Vol. 66, No. 4, 2005, p. 207-212.

Research output: Contribution to journalArticle

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AU - Klekner, A.

AU - Felszeghy, S.

AU - Tammi, R.

AU - Tammi, M.

AU - Csécsei, G.

AU - Módis, L.

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N2 - Object: Hyaluronan (HA) is a highly hydrated macromolecule; it is one of the essential components of the extracellular matrix (ECM) of the arteries and plays an important role in maintaining the biomechanical features of blood vessels. Although the potential contribution of HA in aneurysms of different vessels has been studied intensively, no data are available about the alteration of the HA content in the extracellular matrix of intracranial aneurysms. The aim of the study was to determine the hyaluronan content in the wall of human cerebral arteries. Methods: A biotinylated aggrecan fragment that binds specifically to HA was used to stain samples from cerebral aneurysms (n = 11) to compare the HA content to non-aneurysmal arteries of patients who had intracranial aneurysm (n = 11), and to histologically normal arteries of patients who had expired from non-vascular diseases (n = 14). Digital microscopic densitometry was used for the quantitative analysis of the hyaluronan content in these samples. Results: The highest level (169.5 ±7.9) was detected in aneurysms, while the HA-level of non-aneurysmal vessels was lower (130.2 ± 16.8). Both vessel groups contained significantly higher HA than the normal cerebral arteries (32.9 ± 2.1). Conclusions: Results suggest that an elevated hyaluronan level in the extracellular matrix may affect the cerebral arterial wall architecture. It is reasonable to suppose that the increased hyaluronan content creates a viscoelastic ECM which might improve the biomechanical resistance of the thinned vessel wall.

AB - Object: Hyaluronan (HA) is a highly hydrated macromolecule; it is one of the essential components of the extracellular matrix (ECM) of the arteries and plays an important role in maintaining the biomechanical features of blood vessels. Although the potential contribution of HA in aneurysms of different vessels has been studied intensively, no data are available about the alteration of the HA content in the extracellular matrix of intracranial aneurysms. The aim of the study was to determine the hyaluronan content in the wall of human cerebral arteries. Methods: A biotinylated aggrecan fragment that binds specifically to HA was used to stain samples from cerebral aneurysms (n = 11) to compare the HA content to non-aneurysmal arteries of patients who had intracranial aneurysm (n = 11), and to histologically normal arteries of patients who had expired from non-vascular diseases (n = 14). Digital microscopic densitometry was used for the quantitative analysis of the hyaluronan content in these samples. Results: The highest level (169.5 ±7.9) was detected in aneurysms, while the HA-level of non-aneurysmal vessels was lower (130.2 ± 16.8). Both vessel groups contained significantly higher HA than the normal cerebral arteries (32.9 ± 2.1). Conclusions: Results suggest that an elevated hyaluronan level in the extracellular matrix may affect the cerebral arterial wall architecture. It is reasonable to suppose that the increased hyaluronan content creates a viscoelastic ECM which might improve the biomechanical resistance of the thinned vessel wall.

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