Quantitative autoradiographic analysis of the distribution of [3H]muscimol binding to GABA receptors in chick brain

Michael G. Stewart, Rachel C. Bourne, Jolanta Chmielowska, M. Kálmán, A. Csillág, David Stanford

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Quantitative receptor autoradiography was used to investigate the distribution of high-affinity GABA receptors (GABAA) in left and right hemispheres of the brains of 3-week-old chicks. The receptors were labelled with the potent GABA agonist [3H]muscimol. High levels of [3H]muscimol labelling were found throughout the fore-, mid-, and hindbrain, though considerable variation was found in different regions. In the telencephalon the highest concentration of specific binding was found in the hyperstriatum ventrale followed by the neostriatum, and then the locus parolfactorius of the paleostriatal complex, whilst in the diencephalon highest levels of labelling were present in the infundibulum. In the midbrain distinct lamination was observed in the high levels of [3H]muscimol binding in the optic tectum and in the hind brain the highest density of labelling occurred in the granular layers of the cerebellum. Levels of labelling were generally low in the brainstem regions. The distribution of [3H]muscimol binding sites in good agreement with our previous work on the distribution of GABA-immunoreactivity in the chick brain.

Original languageEnglish
Pages (from-to)387-391
Number of pages5
JournalBrain Research
Volume456
Issue number2
DOIs
Publication statusPublished - Jul 26 1988

Fingerprint

Muscimol
GABA Receptors
Rhombencephalon
Brain
Mesencephalon
GABA Agonists
Neostriatum
Diencephalon
Telencephalon
Superior Colliculi
Pituitary Gland
Autoradiography
Cerebellum
gamma-Aminobutyric Acid
Brain Stem
Binding Sites

Keywords

  • Chick brain
  • Quantitative autoradiography
  • [H]muscimol binding

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Quantitative autoradiographic analysis of the distribution of [3H]muscimol binding to GABA receptors in chick brain. / Stewart, Michael G.; Bourne, Rachel C.; Chmielowska, Jolanta; Kálmán, M.; Csillág, A.; Stanford, David.

In: Brain Research, Vol. 456, No. 2, 26.07.1988, p. 387-391.

Research output: Contribution to journalArticle

Stewart, Michael G. ; Bourne, Rachel C. ; Chmielowska, Jolanta ; Kálmán, M. ; Csillág, A. ; Stanford, David. / Quantitative autoradiographic analysis of the distribution of [3H]muscimol binding to GABA receptors in chick brain. In: Brain Research. 1988 ; Vol. 456, No. 2. pp. 387-391.
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