Quantitative analysis of the postnatal development of Purkinje neurons in the cerebellum of the cat

Cs Vastagh, J. Víg, J. Takács, J. Hámori

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3 Citations (Scopus)

Abstract

We have studied the postnatal quantitative changes of cortical Purkinje neurons in the cerebellum of the cat at the following postnatal groups of age: P0, P42, P72 and adults. An unbiased counting method, the optical fractionator was used for the estimation of Purkinje cell numbers. A significant increase of Purkinje cell number was found between P0 (1.097 × 106) and P42/P72 (1.805 × 106 and 1.895 × 106) declining to 1.429 × 106 in the adult, still 30% higher than in the newborn. It was also observed that during the first few postnatal weeks large "gaps" were present in the Purkinje monolayer as revealed by Nissl staining and metabotropic glutamate receptor 1α immunocytochemistry. These Purkinje cell gaps were observed most frequently in well-definable areas, especially in the intermediate zone of the neocerebellum. Simultaneously with the numerical increase of Purkinje neurons between the P0 and P72 age groups, these gaps disappeared after the third postnatal week resulting in the completion of the Purkinje monolayer in the whole cerebellum.

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalInternational Journal of Developmental Neuroscience
Volume23
Issue number1
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Purkinje Cells
Cerebellum
Cats
Age Groups
Cell Count
Immunohistochemistry
Newborn Infant
Staining and Labeling

Keywords

  • Cerebellum
  • Ganglionic layer
  • Neuronal number

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

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abstract = "We have studied the postnatal quantitative changes of cortical Purkinje neurons in the cerebellum of the cat at the following postnatal groups of age: P0, P42, P72 and adults. An unbiased counting method, the optical fractionator was used for the estimation of Purkinje cell numbers. A significant increase of Purkinje cell number was found between P0 (1.097 × 106) and P42/P72 (1.805 × 106 and 1.895 × 106) declining to 1.429 × 106 in the adult, still 30{\%} higher than in the newborn. It was also observed that during the first few postnatal weeks large {"}gaps{"} were present in the Purkinje monolayer as revealed by Nissl staining and metabotropic glutamate receptor 1α immunocytochemistry. These Purkinje cell gaps were observed most frequently in well-definable areas, especially in the intermediate zone of the neocerebellum. Simultaneously with the numerical increase of Purkinje neurons between the P0 and P72 age groups, these gaps disappeared after the third postnatal week resulting in the completion of the Purkinje monolayer in the whole cerebellum.",
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AU - Víg, J.

AU - Takács, J.

AU - Hámori, J.

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N2 - We have studied the postnatal quantitative changes of cortical Purkinje neurons in the cerebellum of the cat at the following postnatal groups of age: P0, P42, P72 and adults. An unbiased counting method, the optical fractionator was used for the estimation of Purkinje cell numbers. A significant increase of Purkinje cell number was found between P0 (1.097 × 106) and P42/P72 (1.805 × 106 and 1.895 × 106) declining to 1.429 × 106 in the adult, still 30% higher than in the newborn. It was also observed that during the first few postnatal weeks large "gaps" were present in the Purkinje monolayer as revealed by Nissl staining and metabotropic glutamate receptor 1α immunocytochemistry. These Purkinje cell gaps were observed most frequently in well-definable areas, especially in the intermediate zone of the neocerebellum. Simultaneously with the numerical increase of Purkinje neurons between the P0 and P72 age groups, these gaps disappeared after the third postnatal week resulting in the completion of the Purkinje monolayer in the whole cerebellum.

AB - We have studied the postnatal quantitative changes of cortical Purkinje neurons in the cerebellum of the cat at the following postnatal groups of age: P0, P42, P72 and adults. An unbiased counting method, the optical fractionator was used for the estimation of Purkinje cell numbers. A significant increase of Purkinje cell number was found between P0 (1.097 × 106) and P42/P72 (1.805 × 106 and 1.895 × 106) declining to 1.429 × 106 in the adult, still 30% higher than in the newborn. It was also observed that during the first few postnatal weeks large "gaps" were present in the Purkinje monolayer as revealed by Nissl staining and metabotropic glutamate receptor 1α immunocytochemistry. These Purkinje cell gaps were observed most frequently in well-definable areas, especially in the intermediate zone of the neocerebellum. Simultaneously with the numerical increase of Purkinje neurons between the P0 and P72 age groups, these gaps disappeared after the third postnatal week resulting in the completion of the Purkinje monolayer in the whole cerebellum.

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