Quantitation of tissue transglutaminase by a sandwich ELISA system

L. Fesus, Gabriella Arato

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A sandwich ELISA system has been developed to quantitate transglutaminase in human cell extracts. It utilizes affinity-purified rabbit anti-human transglutaminase as the capture antibody and a mouse monoclonal anti-transglutaminase (Birckbichler et al., 1985) as the indicator antibody (together with peroxidase-labeled anti-mouse immunoglobulin). The sensitivity of the assay was less than 1.0 ng/mg cellular protein. Significantly higher concentrations of the enzyme were found in resting versus proliferating or transformed fibroblasts in good agreement with previous activity measurements. The levels of transglutaminase in normal T and B lymphocytes, malignant lymphoid cells and monocytes were also determined.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalJournal of Immunological Methods
Volume94
Issue number1-2
DOIs
Publication statusPublished - Nov 20 1986

Keywords

  • ELISA, sandwich
  • Fibroblast, human
  • Lymphocyte
  • Malignant cell
  • Monoclonal antibody
  • Polyclonal antibody
  • Transglutaminase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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