Quantitation of protein phosphatase 1 and 2A in extracts of the budding yeast and fission yeast

Ilona Farkas, Éva Bakó, Andrea Murányi, Tamás Zeke, M. Sipiczki, P. Gergely

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Abstract

Serine/threonine protein phosphatases are also involved in the control of cell division. The aim of the present study was to compare the activity of protein phosphatase 1 (PP1) and 2A (PP2A) in cell extracts of the budding and fission yeast, made at different phases of growth. The activities of PP1 and PP2A toward phosphorylase were similar in extracts of S. cerevisiae. In S. pombe extracts, PP1 was responsible for more than 80% of the phosphorylase phosphatase activity. Ammonium sulfate-ethanol treatment increased the specific activity of the phosphatases and the percentage of PP2A in S. cerevisiae extracts. No increase in the proportion of PP2A was observed upon the same treatment of S. pombe extracts. The above results were confirmed by fractionation of PP1 and PP2A activities on a heparin-Sepharose column. The proportion of PP1 and PP2A activities did not change significantly during exponential cell growth but cells from stationary phase exhibited lower phosphatase activities. These results may indicate a lower level of expression of the PP2A genes in S. pombe and/or differences in the structure of the holoenzymes or their regulators in the two genera.

Original languageEnglish
Pages (from-to)767-773
Number of pages7
JournalInternational Journal of Biochemistry and Cell Biology
Volume27
Issue number8
Publication statusPublished - Aug 1995

Fingerprint

Protein Phosphatase 1
Protein Phosphatase 2
Saccharomycetales
Schizosaccharomyces
Yeast
Phosphoric Monoester Hydrolases
Saccharomyces cerevisiae
Phosphorylase Phosphatase
Schizosaccharomyces pombe Proteins
Phosphorylases
Holoenzymes
Phosphoprotein Phosphatases
Ammonium Sulfate
Cell growth
Fractionation
Growth
Cell Extracts
Cell Division
Ethanol
Genes

Keywords

  • Heparin-Sepharose chromatography
  • Okadaic acid
  • Protein phosphatase 1 2A
  • Schizosaccharomyces pombe)
  • Yeast (Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry

Cite this

Quantitation of protein phosphatase 1 and 2A in extracts of the budding yeast and fission yeast. / Farkas, Ilona; Bakó, Éva; Murányi, Andrea; Zeke, Tamás; Sipiczki, M.; Gergely, P.

In: International Journal of Biochemistry and Cell Biology, Vol. 27, No. 8, 08.1995, p. 767-773.

Research output: Contribution to journalArticle

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