Quantification of the Reduced Form of Coenzyme Q10, Ubiquinol, in Dietary Supplements with HPLC-ESI-MS/MS

Andrea Vass, Edit Deák, M. Dernovics

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Presence of reduced ubiquinone, ubiquinol, in dietary supplements can originate either from the reduction of ubiquinone due to the co-formulation processes with reducing agents or intentional addition of ubiquinol. As the amount of ubiquinol influences the analytical techniques intended for total ubiquinone determination and the positioning of dietary supplements, the quantification of ubiquinol can be a prerequisite in the quality control process of the related products. In our study, a comprehensive method development has been carried out including (i) the production of ubiquinol together with its HPLC-ESI-MS/MS based verification, (ii) the monitoring of ubiquinol oxidation during the extraction process, (iii) a novel spiking method to validate ubiquinol calibration and (iv) the determination of analyte recovery with an HPLC-UV technique. The combined method was used to quantify ubiquinol in 12 dietary supplement samples containing 31.5–104.5 mg total Q10 g−1. The range of ubiquinol content was 5–16 % related to total ubiquinone concentration and the extraction efficiency values exceeded 99.6 % in all cases.

Original languageEnglish
Pages (from-to)452-458
Number of pages7
JournalFood Analytical Methods
Volume8
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

coenzyme Q10
Dietary supplements
Coenzymes
ubiquinones
Dietary Supplements
quantification
supplement
dietary supplements
high performance liquid chromatography
High Pressure Liquid Chromatography
Ubiquinone
control process
quality control
reducing agents
Reducing agents
methodology
monitoring
analytical methods
Quality control
efficiency

Keywords

  • Dietary supplement
  • Mass spectrometry
  • Recovery
  • Spiking
  • Ubiquinol

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology
  • Safety, Risk, Reliability and Quality
  • Safety Research

Cite this

Quantification of the Reduced Form of Coenzyme Q10, Ubiquinol, in Dietary Supplements with HPLC-ESI-MS/MS. / Vass, Andrea; Deák, Edit; Dernovics, M.

In: Food Analytical Methods, Vol. 8, No. 2, 2014, p. 452-458.

Research output: Contribution to journalArticle

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