Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification

Shuxun Shao, Xiubo Mi, Laurent Ouerdane, Ryszard Lobinski, Juan Francisco García-Reyes, Antonio Molina-Díaz, Andrea Vass, M. Dernovics

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Orthogonal liquid chromatographic (ion exchange, reversed phase, and ion pairing) and mass spectrometric [electrospray ionization (ESI)-TOF-MS, ESI-Orbitrap MS, and inductively coupled plasma mass spectrometry (ICP-MS)] methods were addressed to identify and quantify selenium species from a naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) sample after proteolytic digestion. While selenomethionine (10.1 mg/kg as Se) and selenate (9.5 mg/kg as Se) could be quantified in a straightforward way by anion exchange LC-ICP-MS technique, a multistep purification protocol was required to identify Se-methylselenocysteine and γ-glutamyl-Se-methylselenocysteine in an unambiguous way prior to quantification by using either in-source fragmentation (LC-ESI-TOF-MS) or collision-induced dissociation (LC-ESI-Orbitrap MS). Finally, Se-methylselenocysteine (2.6 mg/kg as Se) and γ-glutamyl-Se-methylselenocysteine (1.2 mg/kg as Se) could contribute to the overall selenium recovery of 72 %. This sample is the first of the Faboideae subfamily and Phaseolus ssp. to be speciated to such an extent for selenium including γ-glutamyl-Se-methylselenocysteine, a highly potential selenium species, which makes this bean material an ideal candidate for functional food purposes.

Original languageEnglish
Pages (from-to)1147-1157
Number of pages11
JournalFood Analytical Methods
Volume7
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Electrospray ionization
Phaseolus
green beans
Selenium
quantification
ionization
Phaseolus vulgaris
chemical derivatives
High Pressure Liquid Chromatography
selenium
Derivatives
Inductively coupled plasma mass spectrometry
fragmentation
atomic absorption spectrometry
Mass Spectrometry
Ion exchange
candidacy
Selenic Acid
food
Selenomethionine

Keywords

  • Orbitrap
  • Phaseolus
  • Selenium
  • Speciation
  • γ-Glutamyl-Se-methylselenocysteine

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology
  • Safety, Risk, Reliability and Quality
  • Safety Research

Cite this

Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification. / Shao, Shuxun; Mi, Xiubo; Ouerdane, Laurent; Lobinski, Ryszard; García-Reyes, Juan Francisco; Molina-Díaz, Antonio; Vass, Andrea; Dernovics, M.

In: Food Analytical Methods, Vol. 7, No. 5, 2014, p. 1147-1157.

Research output: Contribution to journalArticle

Shao, Shuxun ; Mi, Xiubo ; Ouerdane, Laurent ; Lobinski, Ryszard ; García-Reyes, Juan Francisco ; Molina-Díaz, Antonio ; Vass, Andrea ; Dernovics, M. / Quantification of Se-Methylselenocysteine and Its γ-Glutamyl Derivative from Naturally Se-Enriched Green Bean (Phaseolus vulgaris vulgaris) After HPLC-ESI-TOF-MS and Orbitrap MSn-Based Identification. In: Food Analytical Methods. 2014 ; Vol. 7, No. 5. pp. 1147-1157.
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AB - Orthogonal liquid chromatographic (ion exchange, reversed phase, and ion pairing) and mass spectrometric [electrospray ionization (ESI)-TOF-MS, ESI-Orbitrap MS, and inductively coupled plasma mass spectrometry (ICP-MS)] methods were addressed to identify and quantify selenium species from a naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) sample after proteolytic digestion. While selenomethionine (10.1 mg/kg as Se) and selenate (9.5 mg/kg as Se) could be quantified in a straightforward way by anion exchange LC-ICP-MS technique, a multistep purification protocol was required to identify Se-methylselenocysteine and γ-glutamyl-Se-methylselenocysteine in an unambiguous way prior to quantification by using either in-source fragmentation (LC-ESI-TOF-MS) or collision-induced dissociation (LC-ESI-Orbitrap MS). Finally, Se-methylselenocysteine (2.6 mg/kg as Se) and γ-glutamyl-Se-methylselenocysteine (1.2 mg/kg as Se) could contribute to the overall selenium recovery of 72 %. This sample is the first of the Faboideae subfamily and Phaseolus ssp. to be speciated to such an extent for selenium including γ-glutamyl-Se-methylselenocysteine, a highly potential selenium species, which makes this bean material an ideal candidate for functional food purposes.

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