Quantification of non-QB-reducing centers in leaves using a far-red pre-illumination

G. Schansker, Reto J. Strasser

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

An alternative approach to quantification of the contribution of non-Q B -reducing centers to Chl a fluorescence induction curve is proposed. The experimental protocol consists of a far-red pre-illumination followed by a strong red pulse to determine the fluorescence rise kinetics. The far-red pre-illumination induces an increase in the initial fluorescence level (F25 μs ) that saturates at low light intensities indicating that no light intensity-dependent accumulation of Q A - occurs. Far-red light-dose response curves for the F25 μs -increase give no indication of superimposed period-4 oscillations. F25 μs -dark-adaptation kinetics following a far-red pre-pulse, reveal two components: a faster one with a half-time of a few seconds and a slower component with a half-time of around 100 s. The faster phase is due to the non-Q B -reducing centers that re-open by recombination between Q A - and the S-states on the donor side. The slower phase is due to the recombination between Q B - and the donor side in active PS II reaction centers. The pre-illumination-induced increase of the F 25 μs -level represents about 4-5% of the variable fluorescence for pea leaves (∼2.5% equilibrium effect and 1.8-3.0% non-Q B -reducing centers). For the other plant species tested these values were very similar. The implications of these values will be discussed.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalPhotosynthesis Research
Volume84
Issue number1-3
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Lighting
lighting
Fluorescence
fluorescence
Light
Genetic Recombination
light intensity
leaves
legumes
Dark Adaptation
dark adaptation
kinetics
Kinetics
far-red light
Peas
Dosimetry
dose response
oscillation
peas

Keywords

  • Far-red light
  • Non-Q -reducing centers
  • OJIP-transient
  • Plant species dependence
  • Q -Q equilibrium effect

ASJC Scopus subject areas

  • Plant Science

Cite this

Quantification of non-QB-reducing centers in leaves using a far-red pre-illumination. / Schansker, G.; Strasser, Reto J.

In: Photosynthesis Research, Vol. 84, No. 1-3, 06.2005, p. 145-151.

Research output: Contribution to journalArticle

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