Quality control of Hypericum perforatum L. analytical challenges and recent progress

Anastasia Agapouda, Anthony Booker, Tivadar Kiss, J. Hohmann, Michael Heinrich, Dezso a. Csupor

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives: The most widely applied qualitative and quantitative analytical methods in the quality control of Hypericum perforatum extracts will be reviewed, including routine analytical tools and most modern approaches. Key findings: Biologically active components of H. perforatum are chemically diverse; therefore, different chromatographic and detection methods are required for the comprehensive analysis of St. John's wort extracts. Naphthodianthrones, phloroglucinols and flavonoids are the most widely analysed metabolites of this plant. For routine quality control, detection of major compounds belonging to these groups seems to be sufficient; however, closer characterization requires the detection of minor compounds as well. Conclusions: TLC and HPTLC are basic methods in the routine analysis, whereas HPLC-DAD is the most widely applied method for quantitative analysis due to its versatility. LC-MS is gaining importance in pharmacokinetic studies due to its sensitivity. Modern approaches, such as DNA barcoding, NIRS and NMR metabolomics, may offer new possibilities for the more detailed characterization of secondary metabolite profile of H. perforatum extracts.

Original languageEnglish
JournalJournal of Pharmacy and Pharmacology
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Hypericum
Quality Control
Phloroglucinol
Metabolomics
Flavonoids
Pharmacokinetics
High Pressure Liquid Chromatography
DNA

Keywords

  • HPLC
  • Hypericum perforatum
  • DNA barcoding
  • NMR metabolomics

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Quality control of Hypericum perforatum L. analytical challenges and recent progress. / Agapouda, Anastasia; Booker, Anthony; Kiss, Tivadar; Hohmann, J.; Heinrich, Michael; Csupor, Dezso a.

In: Journal of Pharmacy and Pharmacology, 2017.

Research output: Contribution to journalArticle

Agapouda, Anastasia ; Booker, Anthony ; Kiss, Tivadar ; Hohmann, J. ; Heinrich, Michael ; Csupor, Dezso a. / Quality control of Hypericum perforatum L. analytical challenges and recent progress. In: Journal of Pharmacy and Pharmacology. 2017.
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