Qualitative chemical characterization and multidirectional biological investigation of leaves and bark extracts of Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae)

Giustino Orlando, Claudio Ferrante, Gokhan Zengin, Kouadio Ibrahime Sinan, Kouadio Bene, Alina Diuzheva, József Jekő, Zoltán Cziáky, Simonetta Di Simone, Lucia Recinella, Annalisa Chiavaroli, Sheila Leone, Luigi Brunetti, Carene Marie Nancy Picot-Allain, Mohamad Fawzi Mahomoodally, Luigi Menghini

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Abstract

Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae) has a long history of use by folk populations for the management of multiple human ailments. Based on the published literature, there has been no attempt to conduct a comparative assessment of the biological activity and the phytochemical profiles of the leaves and stem bark of A. leiocarpus extracted using methanol, ethyl acetate, and water. By high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn) analysis, quinic, shikimic, gallic, and protocatechuic acids were tentatively identified from all the extracts, while chlorogenic, caffeic, ferulic, and dodecanedioic acids were only characterised from the leaves extracts. Additionally, a pharmacological study was carried out to evaluate potential protective effects that are induced by the extracts in rat colon and colon cancer HCT116 cell line. In general, the methanol and water extracts of A. leiocarpus leaves and stem bark showed potent radical scavenging and reducing properties. It was noted that the stem bark extracts were more potent antioxidants as compared to the leaves extracts. The methanol extract of A. leiocarpus leaves showed the highest acetyl (4.68 mg galantamine equivalent/g) and butyryl (4.0 mg galantamine equivalent/g) cholinesterase inhibition. Among ethyl acetate extracts, the pharmacological investigation suggested stem bark ethyl acetate extracts to be the most promising. This extract revealed ability to protect rat colon from lipopolysaccharide-induced oxidative stress, without exerting promoting effects on HCT116 cell line viability and migration. As a conclusion, A. leiocarpus represents a potential source of bioactive compounds in the development of novel therapeutic agents.

Original languageEnglish
Article number343
JournalAntioxidants
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 2019

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Keywords

  • Anogeissus
  • Antioxidant
  • Bioactive compounds
  • Enzyme inhibition
  • Ulcerative colitis

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Orlando, G., Ferrante, C., Zengin, G., Sinan, K. I., Bene, K., Diuzheva, A., Jekő, J., Cziáky, Z., Di Simone, S., Recinella, L., Chiavaroli, A., Leone, S., Brunetti, L., Picot-Allain, C. M. N., Mahomoodally, M. F., & Menghini, L. (2019). Qualitative chemical characterization and multidirectional biological investigation of leaves and bark extracts of Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae). Antioxidants, 8(9), [343]. https://doi.org/10.3390/antiox8090343