QTL analysis of behavior in nine-spined sticklebacks (Pungitius pungitius)

Veronika N. Laine, G. Herczeg, Takahito Shikano, Johanna Vilkki, Juha Merilä

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The genetic architecture of behavioral traits is yet relatively poorly understood in most non-model organisms. Using an F2-intercross (n = 283 offspring) between behaviorally divergent nine-spined stickleback (Pungitius pungitius) populations, we tested for and explored the genetic basis of different behavioral traits with the aid of quantitative trait locus (QTL) analyses based on 226 microsatellite markers. The behaviors were analyzed both separately (viz. feeding activity, risk-taking and exploration) and combined in order to map composite behavioral type. Two significant QTL - explaining on average 6 % of the phenotypic variance - were detected for composite behavioral type on the experiment-wide level, located on linkage groups 3 and 8. In addition, several suggestive QTL located on six other linkage groups were detected on the chromosome-wide level. Apart from providing evidence for the genetic basis of behavioral variation, the results provide a good starting point for finer-scale analyses of genetic factors influencing behavioral variation in the nine-spined stickleback.

Original languageEnglish
Pages (from-to)77-88
Number of pages12
JournalBehavior Genetics
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

Pungitius pungitius
Smegmamorpha
Quantitative Trait Loci
Gasterosteidae
Behavioral Genetics
quantitative trait loci
linkage groups
Risk-Taking
Microsatellite Repeats
Statistical Factor Analysis
Chromosomes
phenotypic variation
chromosome
microsatellite repeats
chromosomes
Population
analysis
organisms
experiment

Keywords

  • Behavior
  • Fish
  • Microsatellite
  • Personality
  • QTL
  • Stickleback

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Ecology, Evolution, Behavior and Systematics

Cite this

QTL analysis of behavior in nine-spined sticklebacks (Pungitius pungitius). / Laine, Veronika N.; Herczeg, G.; Shikano, Takahito; Vilkki, Johanna; Merilä, Juha.

In: Behavior Genetics, Vol. 44, No. 1, 01.2014, p. 77-88.

Research output: Contribution to journalArticle

Laine, Veronika N. ; Herczeg, G. ; Shikano, Takahito ; Vilkki, Johanna ; Merilä, Juha. / QTL analysis of behavior in nine-spined sticklebacks (Pungitius pungitius). In: Behavior Genetics. 2014 ; Vol. 44, No. 1. pp. 77-88.
@article{66f8825c1815408484c2250189b105b0,
title = "QTL analysis of behavior in nine-spined sticklebacks (Pungitius pungitius)",
abstract = "The genetic architecture of behavioral traits is yet relatively poorly understood in most non-model organisms. Using an F2-intercross (n = 283 offspring) between behaviorally divergent nine-spined stickleback (Pungitius pungitius) populations, we tested for and explored the genetic basis of different behavioral traits with the aid of quantitative trait locus (QTL) analyses based on 226 microsatellite markers. The behaviors were analyzed both separately (viz. feeding activity, risk-taking and exploration) and combined in order to map composite behavioral type. Two significant QTL - explaining on average 6 {\%} of the phenotypic variance - were detected for composite behavioral type on the experiment-wide level, located on linkage groups 3 and 8. In addition, several suggestive QTL located on six other linkage groups were detected on the chromosome-wide level. Apart from providing evidence for the genetic basis of behavioral variation, the results provide a good starting point for finer-scale analyses of genetic factors influencing behavioral variation in the nine-spined stickleback.",
keywords = "Behavior, Fish, Microsatellite, Personality, QTL, Stickleback",
author = "Laine, {Veronika N.} and G. Herczeg and Takahito Shikano and Johanna Vilkki and Juha Meril{\"a}",
year = "2014",
month = "1",
doi = "10.1007/s10519-013-9624-8",
language = "English",
volume = "44",
pages = "77--88",
journal = "Behavior Genetics",
issn = "0001-8244",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - QTL analysis of behavior in nine-spined sticklebacks (Pungitius pungitius)

AU - Laine, Veronika N.

AU - Herczeg, G.

AU - Shikano, Takahito

AU - Vilkki, Johanna

AU - Merilä, Juha

PY - 2014/1

Y1 - 2014/1

N2 - The genetic architecture of behavioral traits is yet relatively poorly understood in most non-model organisms. Using an F2-intercross (n = 283 offspring) between behaviorally divergent nine-spined stickleback (Pungitius pungitius) populations, we tested for and explored the genetic basis of different behavioral traits with the aid of quantitative trait locus (QTL) analyses based on 226 microsatellite markers. The behaviors were analyzed both separately (viz. feeding activity, risk-taking and exploration) and combined in order to map composite behavioral type. Two significant QTL - explaining on average 6 % of the phenotypic variance - were detected for composite behavioral type on the experiment-wide level, located on linkage groups 3 and 8. In addition, several suggestive QTL located on six other linkage groups were detected on the chromosome-wide level. Apart from providing evidence for the genetic basis of behavioral variation, the results provide a good starting point for finer-scale analyses of genetic factors influencing behavioral variation in the nine-spined stickleback.

AB - The genetic architecture of behavioral traits is yet relatively poorly understood in most non-model organisms. Using an F2-intercross (n = 283 offspring) between behaviorally divergent nine-spined stickleback (Pungitius pungitius) populations, we tested for and explored the genetic basis of different behavioral traits with the aid of quantitative trait locus (QTL) analyses based on 226 microsatellite markers. The behaviors were analyzed both separately (viz. feeding activity, risk-taking and exploration) and combined in order to map composite behavioral type. Two significant QTL - explaining on average 6 % of the phenotypic variance - were detected for composite behavioral type on the experiment-wide level, located on linkage groups 3 and 8. In addition, several suggestive QTL located on six other linkage groups were detected on the chromosome-wide level. Apart from providing evidence for the genetic basis of behavioral variation, the results provide a good starting point for finer-scale analyses of genetic factors influencing behavioral variation in the nine-spined stickleback.

KW - Behavior

KW - Fish

KW - Microsatellite

KW - Personality

KW - QTL

KW - Stickleback

UR - http://www.scopus.com/inward/record.url?scp=84893691551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893691551&partnerID=8YFLogxK

U2 - 10.1007/s10519-013-9624-8

DO - 10.1007/s10519-013-9624-8

M3 - Article

C2 - 24190427

AN - SCOPUS:84893691551

VL - 44

SP - 77

EP - 88

JO - Behavior Genetics

JF - Behavior Genetics

SN - 0001-8244

IS - 1

ER -