Pyrimidin receptor funkcio a kozponti idegrendszerben

Translated title of the contribution: Pyrimidoceptor function in the central nervous system

E. Szárics, Ilona Kovács, Richárd Kovács, Nina Skuban, J. Kardos

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A spectroscopic method, using fluorescent Ca2+, K+ and Na+ ion indicators in combination with the use of fast-kinetic techniques on the time scale of 0.00004-10 s has been applied to study mechanisms of P2 pyrimidoceptor-mediated signal transduction in brain homogenates. Effects of the known P2 receptor ligands (ATP, α,β-methylene-ATP, UTP, UDP and uridine) and the P1 receptor ligand, adenosine, were compared by measuring the rates of transmembrane Ca2+, K+ and Na+ ion fluxes in resealed plasmalemma fragments and nerve endings from the rat cerebral cortex. In homogenates containing resealed plasmalemma fragments, uridine (0.03-30 μM), but not adenosine, activated two phases of Ca2+ ion influx with onsets of a few ms and hundred ms in a concentration-dependent manner. Also, the activation of the fast-phase Ca2+ ion response by ATP, UDP and α, β- methylene-ATP whereas that of the slow-phase by UTP and UDP were observed with 3 μM concentration of these P2 receptor ligands. In homogenates containing resealed nerve endings, the fast-phase Ca2+ ion response to uridine was absent. UTP, but not uridine and UDP (3 μM), activated a fast K+ ion influx with onset of + ion influx with onset of >0.1 s whereas the influx of Na+ ion to uridine was detectable below 0.01 s. Both nucleotides, ATP and UTP (3 μM), activated fluctuations of transmembrane Na+ ion influx and efflux. By contrast, UDP caused efflux of Na+ ion in the subsecond range of time. Collectively these results suggest that transmembrane cation fluxes mediated by kinetically distinguishable P2U pyrimidoceptor subtypes are different.

Original languageHungarian
Pages (from-to)227-231
Number of pages5
JournalActa Pharmaceutica Hungarica
Volume69
Issue number5
Publication statusPublished - Nov 1999

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Central Nervous System
Ions
Uridine Diphosphate
Uridine
Uridine Triphosphate
Adenosine Triphosphate
Nerve Endings
Ligands
Purinergic P2 Receptors
Purinergic P1 Receptors
Cerebral Cortex
Adenosine
Cations
Signal Transduction
Nucleotides
Brain

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Pyrimidin receptor funkcio a kozponti idegrendszerben. / Szárics, E.; Kovács, Ilona; Kovács, Richárd; Skuban, Nina; Kardos, J.

In: Acta Pharmaceutica Hungarica, Vol. 69, No. 5, 11.1999, p. 227-231.

Research output: Contribution to journalArticle

Szárics, E, Kovács, I, Kovács, R, Skuban, N & Kardos, J 1999, 'Pyrimidin receptor funkcio a kozponti idegrendszerben', Acta Pharmaceutica Hungarica, vol. 69, no. 5, pp. 227-231.
Szárics, E. ; Kovács, Ilona ; Kovács, Richárd ; Skuban, Nina ; Kardos, J. / Pyrimidin receptor funkcio a kozponti idegrendszerben. In: Acta Pharmaceutica Hungarica. 1999 ; Vol. 69, No. 5. pp. 227-231.
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