Pyrimidine deoxyribonucleosides are phosphorylated to triphosphates at low temperatures too, but are not incorporated into DNA or phospholipids

Károly Szikla, Mária Sasvári-Székety, Tatjana Spasokukotskaja, Mária Staub

Research output: Contribution to journalArticle

Abstract

Thymidine (Thd) was phosphorylated to dTTP also at 0°C, both Ehrlich ascites tumor cells and human tonsillar lymphocytes, but was not incorporated into DNA. The uptake and phosphorylation of 14C-Thd into the pools showed regular kinetics (Km 6,6 uM), and the main metabolite was dTTP (75-84%) both at 0°C, and 37°C. Similarly, deoxycytidine (dCyd) was also transported and phosphorylated to nucleotides (76%) at low temperature, but no incorporation into DNA and phospholipid precursor liponucleotides were labeled with 5-3H- dCyd, 51% of the total pool radioactivity was found in liponucleotides. Transport and phosphorylation of deoxynucleosides seem to be tightly coordinated at both temperatures, which processes are directly coupled to membrane-phospholipid and DNA biosynthesis, but only at physiological temperature while they seem 'uncoupled' at low temperature. The fact that nucleoside phosphorylation occurs also at low temperature has implications for several experimental techniques used in cell biology.

Original languageEnglish
Pages (from-to)1657-1668
Number of pages12
JournalNucleosides and Nucleotides
Volume15
Issue number10
DOIs
Publication statusPublished - Jan 1 1996

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Fingerprint Dive into the research topics of 'Pyrimidine deoxyribonucleosides are phosphorylated to triphosphates at low temperatures too, but are not incorporated into DNA or phospholipids'. Together they form a unique fingerprint.

  • Cite this