Purinergic nerves mediate the non-nitrergic relaxation of the human ileum in response to electrical field stimulation

Sarolta Undi, Rita Benkó, Matyas Wolf, Laszlo Illényi, Örs Peter Horváth, Andras Antal, Zsolt Csontos, Andras Vereczkei, Lorand Barthó

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Abstract

There has been no direct functional evidence for a purinergic innervation of the human intestinal muscle. In the present study, the relaxant effects of electrical field stimulation (1 or 10 Hz for 20 s), ATP, and isoprenaline were studied in organ bath experiments on precontracted circular muscle strips of the human ileum. Non-adrenergic, non-cholinergic relaxations in response to electrical field stimulation in the presence of a nitric oxide synthase inhibitor were significantly reduced by the P2 purinoceptor antagonists pyridoxal-phosphate-6-azophenyl-2′,4′-disulphonic acid (PPADS; 50 μM) or suramin (100 μM). A combination of the two antagonists yielded an approximately 70% inhibition at 1 Hz. The relaxant effect of exogenous ATP, but not that of isoprenaline, was inhibited by PPADS + suramin. It is concluded that purinergic nerves (through P2 purinoceptors) play a mediating role in the non-nitrergic relaxation in the human ileum.

Original languageEnglish
Pages (from-to)242-244
Number of pages3
JournalBrain Research Bulletin
Volume71
Issue number1-3
DOIs
Publication statusPublished - Dec 11 2006

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Keywords

  • Human ileum
  • NANC relaxation
  • Neuromuscular transmission
  • P purinoceptor antagonists
  • Purinergic nerves

ASJC Scopus subject areas

  • Neuroscience(all)

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