Purification of plasmid DNA by an improved electrophoretic protocol

E. Szegedi, Gy Váradi, L. Otten

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Plasmid DNA from Escherichia coli was isolated by electroelution carried out in an agarose gel that contains an incorporated dialysis membrane. As the relative mobility of circular plasmid DNA to linear chromosomal DNA increases when the agarose concentration is decreased, we were able to purify plasmids of up to 50 kbp in 0.3% agarose gel in Tris acetate buffer yielding 10-60 μg DNA ml-1 bacterial culture.

Original languageEnglish
Pages (from-to)777-779
Number of pages3
JournalBiotechnology Techniques
Volume11
Issue number11
Publication statusPublished - 1997

Fingerprint

Sepharose
Purification
Plasmids
DNA
Gels
Circular DNA
Tromethamine
Dialysis membranes
Dialysis
Acetates
Escherichia coli
Buffers
Membranes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Purification of plasmid DNA by an improved electrophoretic protocol. / Szegedi, E.; Váradi, Gy; Otten, L.

In: Biotechnology Techniques, Vol. 11, No. 11, 1997, p. 777-779.

Research output: Contribution to journalArticle

Szegedi, E. ; Váradi, Gy ; Otten, L. / Purification of plasmid DNA by an improved electrophoretic protocol. In: Biotechnology Techniques. 1997 ; Vol. 11, No. 11. pp. 777-779.
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