Purification of human chorionic gonadotropin hormone by anion-exchange high-performance liquid chromatography

I. Fellegvári, K. Valkó, G. Váradi, P. Bauer, M. Kramer

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Abstract

A high-performance liquid chromatography (HPLC) method was developed for the purification of 50mg crude human chorionic gonadotropin (HCG) hormone sample in one chromatographic run on a 250×10 mm SERVA DEAE Poliol-Si-500 column. During the 60-minute linear gradient of 0.05 M sodium sulfate, 0.05 M sodium hydrogen sulfate and 0.05% (v/v) acetonitrile in buffer "B" complete separation was accomplished in 40 min. Identity and purity of the fractions were checked by SDS electrophoretic method. The eluted HCG fractions were dialyzed for 24 hours to remove the buffer salts and liophilized. The activity of the hormone did not decreased significantly during the purification procedure.

Original languageEnglish
Pages (from-to)601-604
Number of pages4
JournalChromatographia
Volume27
Issue number11-12
DOIs
Publication statusPublished - Jun 1 1989

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Keywords

  • Anion exchange separation
  • Column liquid chromatography
  • Human chorionic gonadotropin hormone
  • Preparative separations

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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