Purification of fluorescently derivatized n-glycans by magnetic iron nanoparticles

Csaba Váradi, Emőke Sikora, László Vanyorek, Béla Viskolcz

Research output: Contribution to journalArticle

Abstract

A novel glycoanalytical approach was developed in this study for the purification of fluorescently derivatized N-glycans. Polyethylene glycol (PEG) modified iron-nanoparticles were synthetized by the combination of sonochemical treatment and combustion method. The prepared nanomaterials were applied for a systematic clean-up optimization to maximize purification efficiency of 2-AA labelled glycans. PEG 1000 modified iron-oxalate was found to be the most effective for the selective enrichment of serum N-glycans providing high reproducibility. Different acetonitrile percentages for binding and washing steps were also tested to ensure the same relative peak areas compared to the unpurified sample. The generated novel clean-up strategy provides a potential route to use in-house synthetized magnetic nanoparticles for glycan sample preparation.

Original languageEnglish
Article number1480
JournalNanomaterials
Volume9
Issue number10
DOIs
Publication statusPublished - Oct 2019

Keywords

  • Glycosylation
  • Magnetic nanoparticles
  • Sample preparation

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

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