Purification of cytochrome b-561 from bean hypocotyls plasma membrane. Evidence for the presence of two heme centers

Paolo Trost, A. Bérczi, Francesca Sparla, Giorgio Sponza, Beatrice Marzadori, Han Asard, Paolo Pupillo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The high potential, ascorbate-reducible b-type cytochrome of plant plasma membranes, named cytochrome b-561, has been purified to homogeneity from etiolated bean hypocotyls. The pure protein migrated in denaturing electrophoresis as a broad band of approximately 55 kDa, and was found to be glycosylated. Optical redox titrations of partially purified cytochrome b-561 indicated that it contains two hemes with similar spectral features, but distinct midpoint redox potentials (E(m7) + 135 mV and +206 mV, respectively). The presence of two heme centers in cytochrome b-561 is consistent with its role in electron transfer across plant plasma membranes. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalBBA - Biomembranes
Volume1468
Issue number1-2
DOIs
Publication statusPublished - Sep 29 2000

Fingerprint

Hypocotyl
Cell membranes
Heme
Purification
Cell Membrane
Oxidation-Reduction
Cytochrome b Group
Electrophoresis
Titration
Electrons
cytochrome b561
Proteins

Keywords

  • Ascorbate
  • Cytochrome b-561
  • Phaseolus vulgaris
  • Plasma membrane redox
  • Redox titration

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Purification of cytochrome b-561 from bean hypocotyls plasma membrane. Evidence for the presence of two heme centers. / Trost, Paolo; Bérczi, A.; Sparla, Francesca; Sponza, Giorgio; Marzadori, Beatrice; Asard, Han; Pupillo, Paolo.

In: BBA - Biomembranes, Vol. 1468, No. 1-2, 29.09.2000, p. 1-5.

Research output: Contribution to journalArticle

Trost, Paolo ; Bérczi, A. ; Sparla, Francesca ; Sponza, Giorgio ; Marzadori, Beatrice ; Asard, Han ; Pupillo, Paolo. / Purification of cytochrome b-561 from bean hypocotyls plasma membrane. Evidence for the presence of two heme centers. In: BBA - Biomembranes. 2000 ; Vol. 1468, No. 1-2. pp. 1-5.
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