Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa

Kalyani Mahapatra, Ranjan Kumar Nanda, Subhendu Sekher Bag, Rintu Banerjee, Ashok Pandey, G. Szakács

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Tannase (tannin acyl hydrolase EC 3.1.1.20) produced by Aspergillus awamori nakazawa was purified and characterized. Optimal conditions of production were determined using varying substrate combinations and studying fermentation on various media combinations. Fermentation was carried out for 46 h for optimum enzyme production. Enzyme samples were obtained from the broth after fermentation by acetone precipitation of the supernatant followed by gel filtration chromatography. The properties of the enzyme were investigated. The optimum conditions of temperature and pH were investigated and the effects of urea, surfactant and chelator were studied. Tannase from this new isolate exhibited optimum activity at 35°C and at a pH of 5.0. Urea concentrations higher than 3 M were inhibitory. Increasing concentrations of sodium lauryl sulphate also led to decrease in activity. Two percent SLS was inhibitory. Increasing concentrations of EDTA had an inhibitory effect on tannase. Tannase was found to be a glycoprotein. Circular dichroism analysis of purified fractions of tannase indicates that the β-sheet structure in tannase is predominant indicating its globular nature.

Original languageEnglish
Pages (from-to)3251-3254
Number of pages4
JournalProcess Biochemistry
Volume40
Issue number10
DOIs
Publication statusPublished - Oct 2005

Fingerprint

tannase
Tannins
Hydrolases
Aspergillus
Fermentation
Purification
Enzymes
Urea
Glycoproteins
Ethylenediaminetetraacetic acid
Dichroism
Sodium dodecyl sulfate
Chelating Agents
Acetone
Chromatography
Surface-Active Agents
Edetic Acid
Sodium Dodecyl Sulfate
Surface active agents
Gels

Keywords

  • CD
  • Fungal tannase
  • GFC
  • Glycoprotein
  • HPLC
  • Tannase assay
  • Tannase substrate

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Engineering (miscellaneous)
  • Industrial and Manufacturing Engineering

Cite this

Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa. / Mahapatra, Kalyani; Nanda, Ranjan Kumar; Bag, Subhendu Sekher; Banerjee, Rintu; Pandey, Ashok; Szakács, G.

In: Process Biochemistry, Vol. 40, No. 10, 10.2005, p. 3251-3254.

Research output: Contribution to journalArticle

Mahapatra, Kalyani ; Nanda, Ranjan Kumar ; Bag, Subhendu Sekher ; Banerjee, Rintu ; Pandey, Ashok ; Szakács, G. / Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa. In: Process Biochemistry. 2005 ; Vol. 40, No. 10. pp. 3251-3254.
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