Purification and characterization of an extracellular β-D-xylosidase from Aspergillus carbonarius

T. Kiss, L. Kiss

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Abstract

The production of an extracellular β-D-xylosidase (β-D-xyloside xylohydrolase, EC 3.2.1.37) by four Aspergillus strains (A. carbonarius, A. nidulans, A. niger and A. oryzae) grown on wheat bran medium was compared. The highest amount of the enzyme was found in the culture of A. carbonarius. The β-D-xylosidase from A. carbonarius was purified to homogeneity by a rapid procedure, using hydrophobic interaction chromatography, chromatofocusing and affinity chromatography. The purified enzyme possessed not only β-D-xylosidase activity, but also α-L-arabinosidase activity. Mixed substrate experiments revealed that a single active centre was responsible for the splitting of the corresponding synthetic substrates. The molecular weight of the purified enzyme proved to be 100,000 Da, as estimated by SDS-PAGE. The isoelectric point was at pH 4.4. The pH and temperature optima were 4.0 and 60 °C, respectively. The enzyme remained stable over a pH range of 3.5-6.5 and up to 50 °C for 30 min. The Michaelis constant for p-nitrophenyl β-D-xyloside was 0.198 mM. Kinetic studies demonstrated that the lack of the C-5 hydroxylmethyl group and the configuration of the C-4 hydroxyl group on the pyranoside ring play an important role in both substrate binding and splitting.

Original languageEnglish
Article number273204
Pages (from-to)465-470
Number of pages6
JournalWorld Journal of Microbiology and Biotechnology
Volume16
Issue number5
DOIs
Publication statusPublished - Jan 1 2000

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Keywords

  • Aspergillus carbonarius
  • Characterization of β-D-xylosidase
  • Purification of β-D-xylosidase

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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