Purification and Characterization of a Novel Cold-Active Lipase from the Yeast Candida zeylanoides

Iva Čanak, Adrienn Berkics, Nikolett Bajcsi, Monika Kovacs, Agnes Belak, Renata Teparić, A. Maráz, Vladimir Mrša

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cold-active lipases have attracted attention in recent years due to their potential applications in reactions requiring lower temperatures. Both bacterial and fungal lipases have been investigated, each having distinct advantages for particular applications. Among yeasts, cold-active lipases from the genera Candida, Yarrowia, Rhodotorula, and Pichia have been reported. In this paper, biosynthesis and properties of a novel cold-active lipase from Candida zeylanoides isolated from refrigerated poultry meat are described. Heat-sterilized olive oil was found to be the best lipase biosynthesis inducer, while nonionic detergents were not effective. The enzyme was purified to homogeneity using hydrophobic chromatography and its enzymatic properties were tested. Pure enzyme activity at 7°C was about 60% of the maximal activity at 27°C. The enzyme had rather good activity at higher temperatures, as well. Optimal pH of pure lipase was between 7.3 and 8.2, while the enzyme from the crude extract had an optimum pH of about 9.0. The enzyme was sensitive to high ionic strength and lost most of its activity at high salt concentrations. Due to the described properties, cold-active C. zeylanoides lipase has comparative advantages to most similar enzymes with technological applications and may have potential to become an industrially important enzyme.

Original languageEnglish
Pages (from-to)403-411
Number of pages9
JournalJournal of Molecular Microbiology and Biotechnology
Volume25
Issue number6
DOIs
Publication statusPublished - Dec 1 2015

Keywords

  • Candida zeylanoides
  • Cold-active enzyme
  • Cold-active lipase
  • Lipase
  • Lipase properties
  • Lipase purification

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Microbiology

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